Interaction of nanoparticles with proteins: relation to bio-reactivity of the nanoparticle
© Saptarshi et al.; licensee BioMed Central Ltd. 2013
Received: 24 May 2013
Accepted: 12 July 2013
Published: 19 July 2013
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© Saptarshi et al.; licensee BioMed Central Ltd. 2013
Received: 24 May 2013
Accepted: 12 July 2013
Published: 19 July 2013
Interaction of nanoparticles with proteins is the basis of nanoparticle bio-reactivity. This interaction gives rise to the formation of a dynamic nanoparticle-protein corona. The protein corona may influence cellular uptake, inflammation, accumulation, degradation and clearance of the nanoparticles. Furthermore, the nanoparticle surface can induce conformational changes in adsorbed protein molecules which may affect the overall bio-reactivity of the nanoparticle. In depth understanding of such interactions can be directed towards generating bio-compatible nanomaterials with controlled surface characteristics in a biological environment. The main aim of this review is to summarise current knowledge on factors that influence nanoparticle-protein interactions and their implications on cellular uptake.
Nanoparticles (NPs) have unique properties that may be useful in a diverse range of applications, and consequently they have attracted significant interest. Particularly in the bio-medical field, the use of nano vaccines and nano drugs are being intensively researched. Nevertheless, our knowledge about the bio-compatibility and risks of exposure to nanomaterials is limited. Exposure to nanomaterials for humans may be accidental, for example occupational exposure, or intentional, for example in the use of nano-enabled consumer products. There are an increasing number of studies that demonstrate adverse effects of nanomaterials in in-vitro cellular systems, but it is unclear whether the available data can be reliably extrapolated to predict the adverse effects of nanotechnology for humans. Hence, there is an urgent need to understand the molecular mechanisms of nanoparticles-to-biological system interaction.
In a biological medium, NPs may interact with bio-molecules such as proteins, nucleic acids, lipids and even biological metabolites due to their nano-size and large surface-to-mass ratio. Of particular importance is the adsorption of proteins on the nanoparticle surface. The formation of nanoparticle-protein complexes is commonly referred to as the nanoparticle-protein corona (NP-PC). A number of consequences of protein adsorption on the NP surface can be speculated. Overall, the NP-PC can influence the biological reactivity of the NP [1, 2].
This review gives a summary of the current research on the physico-chemical characteristics influencing the formation of the NP-PC, its impact on the structure of adsorbed proteins and the overall implication these interactions have on cellular functions.
Proteins are polypeptides with a defined conformation and carry a net surface charge depending on the pH of the surrounding medium. Adsorption of proteins at the nano-bio interface is aided by several forces such as hydrogen bonds, solvation forces, Van der Waals interactions, etc. The overall NP-PC formation is a multifactorial process and not only depends on the characteristics of the NP, but also on the interacting proteins and the medium. Specific association and dissociation rates for each protein decide longevity of their interaction with the NP surface. Irreversible (or at least long-term) binding of proteins on the NP leads to formation of a “hard corona” whereas quick reversible binding of proteins that have faster exchange rates defines a “soft corona” [2–6].
Serum/plasma cellular proteins represent complex biological systems, and it has to be considered that NPs can form Bio/Nano complexes when exposed to several, very different systems in vivo. An inhaled NP may pass through the mucosal layer, lung epithelial cells and finally enter in to the blood. Similarly, at the cellular level after being phagocytised by a monocyte, the NP may be taken into the endosomes that ultimately fuse with lysosomes. Each of these proteomes represents unique environments and has specific properties with respect to their protein composition, enzymatic activities, pH, ion composition etc. These environments may cause the NP to undergo a complex sequence of modifications that are far from being fully understood. Even within one environment the NP-protein interactions are constantly changing. For example, when exposed to blood plasma the nano-bio interface has been reported to change with time due to constant adsorption and desorption of proteins . NPs that have entered the body thus have to be considered as evolving systems that are shaped by sequential exposure to different protein rich environments. Kinetics of protein adsorption on the NP surface can be influenced by several factors. Amount of proteins available to interact with the NP surface is one such factor that can greatly influence the NP-PC composition. When plasma proteins were applied at concentrations between 3% and 80% of plasma, Monopoli and co-workers observed that the proteins bound to NPs varied with plasma concentration, while relative amounts of some abundant proteins adsorbed on surfaces of silica or polystyrene NPs increased with increasing plasma concentrations . When travelling through different protein rich environments in an in vivo system the NP surface may get pre-coated with specific proteins. This can also determine which new protein will bind to the already formed NP-protein complex. Pre-coating of pulmonary surfactant proteins was shown to influence the subsequent adsorption of plasma proteins on the surface of multi walled carbon nanotubes (MWCNT) . Also, silica or polystyrene NPs were shown to retain a “fingerprint” of plasma proteins even after subsequent incubations with other biological fluids .
Comprehensive overview of serum/plasma proteins adsorbed on the surface of different types of nanomaterials with varied size and surface chemistries
Coagulation factors, Immunoglobulins, Lipoproteins, Complement proteins, Acute phase proteins
Human serum (depleted)
Complement proteins, Plasminogen, Anti-CD4, c4a, Immunoglobulin, Albumin, Complement , Plasminogen
NH2,NHR, NR2,NR3 + COO-, SO− 3, SO− 4
Albumin, Apolipoproteins, Immunoglobulins, Hemoglobin, Haptoglobins
Albumin, Apolipoprotiens, Fibrinogen, Immunoglobulins, C4BPαchain
2 Macroglobulin precursor, Complement factors, plasminogen, Coagulation factors
Albumin, α1Antitrypsin, Fibrinogen chains, Immunoglobulin chains, Transferrin, Transthyretin
5, 10, 20
(PAA) polymer coated
Albumin, Fibrinogen chains, Apolipoprotein A1
15, 40, 80
Transport proteins, Coagulation factors, Tissue development proteins
TiO2 NPs ZnO NPs Quartz sand Carbon nanotubes SiO2 NPs
Silone, alumina, silica coated
Fibrinogen chains, Immunoglobulin light chains, Fibrin, albumin, ApoA1, Complement component proteins, Fibronectin,
8, 20, 25
Immunoglobulins, Lipoproteins, Complement proteins, Coagulation proteins, Acute phase proteins, Cellular proteins, Serum proteins
TiO2 NPs ZnO NPs SiO2 NPs
Albumin, Immunoglobulins, Fibrinogen, Transferrin, Apolipoprotein A1,Complement proteins
Dextran COOH, NH2,PEG COOH, PEG-NH2
Albumin, Apolipoprotein A-1 Complement factors, Vitronectin, Haemoglobin
Adsorption of a protein on the NP surface also depends on the affinity of the protein towards the NP surface and its ability to completely occupy the surface. The way in which protein molecules arrange themselves on the NP surface may affect the biological reactivity of the latter at the cellular level . Plasma proteins such as human serum albumin (HSA) and transferrin were shown to adsorb in a monolayer fashion on iron-platinum (FePt) NP surface . Rezwan et al. observed that bovine serum albumin (BSA) adsorbs on aluminium oxide surface as a monolayer by using 30-36% of its total negative charge and that additional BSA molecules from the medium bind onto this monolayer as dimers .Detailed studies along these lines can be useful in designing protein-conjugated NP surfaces for future applications.
The NP surface can modify the structure and therefore the function of the adsorbed protein thus affecting the overall bio-reactivity of the NP. This section further explores the fate of the proteins bound on the NP surface. Curved NP surfaces compared to planar surfaces provide extra flexibility and enhanced surface area to the adsorbed protein molecules . Curved NP surfaces can also affect the secondary structures of proteins, and in some cases cause irreversible changes . It is interesting to note that chemical properties of individual proteins and their structural flexibility also play an important role in regulating such surface-driven modifications to their secondary structures . Gold NPs were shown to influence conformational changes in the structure of bovine serum albumin (BSA) in a dose-dependent manner , whereas no major conformational change was recorded for BSA when adsorbed to carbon C60 fullerene NP . Titanium dioxide (TiO2) NP were shown to cause conformational change and reduce polymerization of tubulin, which is an essential cytoskeletal protein . Spectroscopic investigation of zinc oxide (ZnO-NP) interaction with BSA also showed no structural perturbation to its overall structure, however, minor conformational changes were reported . An irreversible conformational change in the secondary structure of the protein transferrin was observed upon interaction with SPIONs . NP-induced protein conformational changes may affect the downstream protein-protein interactions, cellular signalling and also DNA transcription, which is particularly important for enzymes. Loss of enzyme activity can result due to the conformational changes in the active site, brought about by the NP surface. SWCNTs were able to differentially induce loss of structure and catalytic activity for two enzymes investigated . Turci et al. showed that RNAse and lysozyme retained their native structures on silica NP while albumin and lactoperoxidase underwent an irreversible conformational change . Likewise, such conformational changes can also increase the accessibility of the enzyme active site for its substrate. Silica NP were able to induce a molten globule-like conformational change in human carbonic anhydrase while removal of the NP resulted in formation of three intermediate native-like conformations each one of which retained catalytic activity. Covalently bound enzymes horse radish peroxidise, subtilisin Carlsberg, and chicken egg white lysozyme on SWCNTs were also shown to retain their activity and their native structure even under denaturing conditions.
NPs can also induce conformational changes in proteins that can lead to fibril formation [37, 38]. Linse et al. showed that a range of NPs (copolymer, ceria, carbon nanotubes, quantum dots) were capable of inducing fibrillation of β2-microglobulindue to increased protein localization on the NP surface, which led to oligomer formation . Fibrillation of proteins is associated with diseases such as Parkinson’s and Alzheimer’s. The fact that NPs can act as platforms to initiate such protein structural changes demands further investigation of this phenomenon.
Summary of literature on proteins subjected to conformational changes upon interaction with nanoparticle surfaces
NP type and size
Protein mol. wt. and size nm (if provided)
Change in protein structure
ZnO NPs (25 nm)
Vibrio cholera Tox r
NP-protein complex susceptible to denaturation
ZnO NPs (N/A)
Minor conformational changes, secondary structure retained
ZnO NPs (N/A)
Minor conformational changes in secondary structure
TiO2 NPs (20 nm)
Protein polymerization affected
SiO2 NPs (~40 nm)
BSA and lactoperoxidase bound irreversibly
Hen egg lysozyme
SiO2 NPs (6,9,15 nm)
Human Carbonic anhydrase
Protein activity was retained
Alumina and hydroxyapatite particles (100-300 nm)
66 kDa 8 × 8 × 3
Loss in α-helical structure
Hen egg lysozyme
14.3 kDa 4.6 × 3 × 3
Bovine serum fibrinogen
350 kDa 6 × 6.5 × 45
Gold (45 nm)
Conformational change was dose dependent
Gold (5-100 nm)
CD and FS
Minor conformational changes observed
Gold (7-22 nm)
340 kDa 45 × 5
Unfolding induced immune response in THP-1 cells
SPIONs (5-10 nm)
Horse radish peroxidase
NP-protein complexes retained enzymatic activity
Chicken egg white lysozyme
Protein adsorption, physical characteristics of the NPs and the properties of interacting cells may influence NP uptake. Kinetics of uptake of the same nanomaterial has been shown to differ with different cell types [50, 51]. Adsorption of proteins on the NP surface can take place almost instantly. Therefore, it can be assumed that interaction of the NP with cellular structures is indirect and occurs mostly via the NP-PC and not the bare NP surface (Figure 2 i). The NP-PC can thus influence the uptake of the NP by the cell. The uptake might be either inhibited due to loss of protein structure of an adsorbed protein, or facilitated due to unfolding of the adsorbed protein to access receptors on the cell surface. This is particularly important when looking at differential binding of physiologically active proteins on the NP. Several in vitro studies have explored cellular uptake of NP in the presence of serum proteins. Dutta et al. carried out an elaborate study to show that albumin adsorbed on the surface on SWCNTs was responsible for inducing an anti-inflammatory pathway in RAW macrophages, thus highlighting that the identity of the adsorbed protein may dictate the bio-reactivity of the NP surface . Similarly, adsorption of the lung protein SP-A on magnetite NPs was also shown to enhance their uptake by macrophages when compared to ones pre-coated with BSA . Caveolae mediated endocytosis of fluorescent polystyrene NPs (20-100 nm) was shown to be dependent on the presence of albumin on the NP surface. Additionally the caveolae that are cell membrane invaginations typically 60-80 nm in diameter were also shown to contain up to three 20 nm and 240 nm polystyrene NPs, which suggested that these structures can be flexible in accommodating larger sized NP-protein complexes .
Apolipoproteins are a class of proteins that are often found in the NP-PC in blood for a number of NP surfaces (Table 1). These have been of interest because of their ability to aid in uptake of NPs by binding to specific receptors on cells. Apolipoproteins B and E were shown to assist in transport of drug bound-polysorbate 80 coated NPs across the blood brain barrier. Receptor mediated endocytosis was speculated to be the means of uptake in this case [55, 56]. The impermeable nature of the blood brain barrier makes it difficult to deliver essential drugs and other compounds to the brain. While the ability of NPs to translocate across this barrier provides a promising future in this direction, it also raises important safety concerns regarding toxicity of nanomaterials.
Certain serum proteins such as immunoglobulins and complement pathway proteins possess opsonising characteristics. The presence or enrichment of such opsonising proteins on the NP surface in blood can lead to immune recognition of the NP-protein complex, otherwise not intended. In one study, uptake of NH2-polystyrene NPs by macrophages in a protein free medium was shown to change from clathrin-mediated endocytosis to phagocytosis when incubated in serum enriched media . Thus showing that opsonisation of the NP surface by serum proteins remarkably influences its uptake. Adsorption of complement protein C3 and opsonising protein IgG on 50 nm lecithin-coated polystyrene NPs was also shown to increase with time and this directly influenced their uptake by murine Kupffer cells .
The large surface area adsorption of proteins on the NP surface often leads to an increase in the hydrodynamic size of the latter. Such large NP-protein complexes can be taken up by phagocytic cell types and also non-phagocytic cells. Lesniak et al. showed that uptake of polystyrene NP by non-phagocytic lung epithelial cells was significantly higher when incubated in non-heated serum compared to NPs incubated in heat inactivated serum . While this study does not state the exact mechanism of uptake the authors report that the amount of protein and also the presence of heat labile complement proteins in the non-heat inactivated serum may be responsible for the observed enhanced uptake.
Another possible explanation for the enhanced uptake is that, cellular interaction of the NP is non-specific; depending exclusively on the amount of protein, rather than the presence of certain proteins on the NP surface as discussed above. This was shown by Ehrenberg et al. in their study where, NPs incubated with complete serum or serum depleted of several abundant proteins did not affect the association of NPs with endothelial cells in vitro. While it is interesting to know that protein binding on the NP surface facilitates its uptake and other interactions, there have been some contradictory reports in the literature. Uptake of FePt NPs by HeLa cells was suppressed in the presence of the NP-PC . Also, silica NPs dispersed in serum free medium were taken up more efficiently by lung epithelial cells as compared to ones in presence of 10% serum. By and large, the amount and identity of the protein adsorbed on the NP surface seems to determine the uptake of the NP.
Apart from protein adsorption other minor differences in physicochemical characteristics such as zeta potential, and size of the NP surface have also been shown to influence their mode of uptake by cells [51, 60–63]. Interestingly, sedimentation capacity was proposed to be the driving factor for uptake of gold NP by human breast cells. This was clearly demonstrated by exposing human breast cancer cells to gold NPs in upright or inverted configurations . Also, Kim and co-workers reported that the phase of cell cycle was an important factor that influenced uptake of 40 nm yellow-green PS-COOH by A549 cells. They also demonstrated that the NP taken up by the parent cells were divided between the daughter cells upon cell division suggesting implications on clearance or accumulation of NPs in vivo.
A review of the literature pertaining to cellular uptake of NPs reveals a number of inconsistencies regarding factors influencing this interaction. It has to be taken into account that most of these studies are conducted under in vitro conditions, often with immortal cell lines which may show different characteristics as compared to their ex vivo counterparts. Moreover, in vivo NPs interact not only with the protein micro-environments but also other cellular moieties simultaneously, making it a challenging task to correctly extrapolate behaviour of NPs in vivo. Perturbation of the native structure of the bound protein depends on the surface of the interacting NP and together these two factors direct the bio-reactivity of the NP.
NP composition [66, 67], hydrophobicity , presence of specific functional groups, pH and temperature  have been shown to affect protein adsorption on the surface of NPs. Sedimentation of NPs especially in an in vitro exposure system has also been reported to influence cellular interactions .
Colloidal solutions of NPs often have a tendency to form agglomerates. NP size, concentration, and surface charge can influence agglomerate formation. Coarse NP agglomerates can exert noticeably different biological properties compared to NPs that are efficiently dispersed [69–71]. Agglomeration can also change the available surface area for protein binding. Uneven surface of the agglomerated NP can induce protein conformational changes. NPs dispersed in protein free media often tend to agglomerate. Studies have reported the use of ultrasonic energy, surfactants , polymer coating  protein containing dispersion media like serum, alveolar lung fluid, etc. to control agglomeration [52, 74–77]. Dispersal of high concentrations of NPs in solutions containing certain proteins such as fibrinogen can in contrast lead to aggregation, due to the formation of inter-particle bridges by the protein [78, 79].
Yet another physical characteristic of the NP known to influence protein binding is size. Twelve nm sized negatively charged poly acrylic acid Au NPs were reported to bind fibrinogen with higher affinity compared to 7 nm NPs . In another study, 15 nm silica NPs induced a six-fold higher change in the secondary structure of human carbonic anhydrase I protein compared to 6 nm silica NPs . Stability of ribonuclease A was dramatically reduced with binding to silica NPs of increasing sizes . Silica NPs (100 nm) were capable of inducing greater loss of structure and function for the protein lysozyme as compared to 4 nm sized particles . On the other hand, Dutta et al. reported that plasma protein adsorption profiles remained uniform for differentially sized silica NPs . This emphasizes again, that it is very difficult to formulate rules about protein interactions that apply to different types of NPs.
The shape of the NP can be important as well. This was confirmed for TiO2 nanorods and nanotubes that differentially adsorbed plasma proteins . Likewise, modification of NP surface charge can also influence protein adsorption. Studies have demonstrated that NP surfaces with no charge bind less proteins than their negatively charged (COOH functionalized) or positively charged (NH2 functionalized) counterparts [18, 82]. Polyethyleneimine-functionalization of (ZnO) NPs favoured their interaction with albumin as compared to pristine ones . A recent study showed that structural modification of a self-protein such as fibrinogen can be affected by the surface properties of gold NPs. Negatively charged gold NPs, unlike positively or neutral charged ones, were shown to bind fibrinogen in an orientation that led to cytokine release in human monocytic THP-1cells in vitro. Influence of the NP surface on immune stimulation by a self- protein is an important concern. Application of this information to extrapolate adverse effects of such interactions under in vivo conditions however needs further study.
Chemical fabrication of the NP surface to avoid adsorption of proteins can be carried out using polyethylene glycol (PEG), also referred to as “PEGylation”. This imparts a “stealth character” to an NP surface, shielding it from being recognized by immune cells . NPs can be made to remain in circulation for longer periods of time by controlling the density of PEG on their surface [83, 84]. Siliconate treatment of the NP surface too has been reported to prevent protein adsorption .
An important issue that requires further attention is the indirect influence of the NP-physico-chemical characteristics on cytotoxicity, cell signalling etc. Cytotoxicity and cytokine release by lung epithelial cells when exposed to ZnO or TiO2nano-powders was influenced by their shape and crystalline forms, respectively . Differential inflammatory potential and cellular association was recorded for spherically shaped or sheets of nano ZnO . Silver nanorods were shown to be toxic to the human lung epithelial cells, while nanospheres with the same mass concentration were not . Thus, the influence of the proteins adsorbed on the NP surface cannot be disregarded completely when assessing immunotoxic outcomes of NPs.
Summary of analytical techniques to conduct physico-chemical characterisation, monitor nanoparticle surface driven protein conformational changes and uptake of nanoparticles by cellular structures
Nanoparticle physical characterisation
Size and charge
Dynamic light scattering
Changes in the hydrodynamic diameter of NP upon binding to proteins
Changes in the hydrodynamic diameter of NP
Inductively coupled mass spectrometry
For detecting elemental composition of the nanomaterial
Shape and structure
X ray diffraction
Determination of crystalline structure
Visualisation of nanoparticle structure
Braunauer Emmet Teller method
Measures specific surface area using adsorption of gas on the surface
Uses sound energy to disrupt large aggregates of NP
Nanoparticle protein interaction
Protein binding affinity
To measure binding constant, thermodynamic parameters of NP-protein interactions
Measures change in fluorescence spectra due to NP-protein interaction
Measures change in absorption spectra due to NP-protein interaction
Quartz crystal balance
Detects change in mass at the oscillating quartz surface due to NP-protein interaction
Surface Plasmon resonance
Detects change in oscillation of electrons on a metal surface due to NP-protein interaction
Atomic force microscopy
Gives surface profile of the nanomaterial
Fluorescence correlation spectroscopy
Binding characteristics depending on fluctuation in florescence
Nanoparticle surface induced protein structure changes
Protein structural changes after binding
Circular Dichroism spectroscopy
Measures changes in secondary structure of proteins depending on chiral properties of proteins
Fourier transformed infrared spectroscopy
Measures adsorption of amide bonds in the proteins to derive structural change
Studies molecular vibrations to predict structure
Nuclear Magnetic Resonance
Relies on magnetic properties of atomic nuclei to predict structure
Nanoparticle- Cellular interactions
Visualization of fluorescent nanoparticles in vitro
Confocal micro Raman spectroscopy
The use of other techniques such as isothermal titration calorimetry, surface plasmon resonance has also been reported to characterise protein binding to various surfaces. A recent study showed that 70 nm copolymer NPs bind around 650 human serum albuminmolecules and 200 nm particles can accommodate up to 4600 molecules, using isothermal calorimetry . Quartz crystal microbalance, a technique that detects change in mass at the oscillating quartz surface due to NP-protein interaction, was employed to probe adsorption of myoglobin, BSA, and cytochrome c proteins on the surface modified gold NPs . The choice of analytical techniques for studying NP-PC greatly depends on the physical state of the NP.
Visualization of NPs uptake by cells is often carried out by florescent labelling of the NP surface or synthesis of fluorescent NPs which can be detected by flow cytometry or confocal laser scanning microscopy. Fluorescent labelling of NPs can however modify its surface thus interfering with subsequent protein interactions. Confocal Raman microscopy was recently used to study uptake of Al2O3 and CeO2 NPs by Lopis and co-workers . This technique was reported to be label-free and specific at the single cell level.
Studies have attempted to propose models to generalize different aspects of NP-protein interactions. Dell’Orco and co-workers formulated a mathematical model to predict kinetics of protein binding to NP surfaces that can be extended to any proteome-NP combination . Nangi et al. developed a simulation model to predict energy barriers, translocation rate constants and half-lives of NPs through lipid membranes as a function of their physical properties . Xia et al. introduced the biological surface adsorption index (BSAI) to predict the order in which factors such as hydrophobicity, hydrogen bonding, polarizability of NP surface and ion-pair electrons affect protein adsorption at the nano-bio interface, using12 physico-chemically defined protein-mimicking probes and MWCNTs . The experimental approach employed by most studies currently involves detailed study of a single protein with the NP surface rather than the complete NP-PC that consists of several different types of proteins. Strategic use and combination of the available analytical techniques is needed to analyse several aspects of NP-protein interactions simultaneously.
Characterization and analysis of proteins bound to the NP surface is the first step towards understanding the true nature of the NP-mediated biological effects. Research thus far highlights that size, shape, and surface characteristics of NPs affect protein adsorption and also have the capability to modify the structure of the adsorbed protein molecules. This can significantly affect the reactivity of the NP with cells and determine the route and efficiency of NP uptake. The adsorbed proteins may also promote translocation of the NP across cellular barriers, and clearance or accumulation in vital organs. Interestingly, most studies conducted in this direction focus on in vitro test systems, therefore, extrapolation of this information in predicting behaviour of NPs in vivo remains a challenging task and needs further investigation. Systematic analysis of binding characteristics of novel NPs with proteins having different structures, shapes and functional properties can enhance our existing knowledge about NP-protein interactions. Thorough understanding of NP-protein interactions might lead to strategic manipulation of NP surfaces to adsorb specific functional proteins or small drug molecules intended for delivery in vivo. Furthermore this knowledge might also prove to be useful in predicting nanotoxicity related safety concerns. In summary, NP-PC dictates the overall biological reactivity of the otherwise inorganic NP surface. Understanding the dynamics of this complex interaction can thus provide useful insights into cytotoxic, inflammatory potential and other key properties of these novel materials that can be explored for developing safer and value added nanomaterials for future applications.
Single walled carbon nanotubes
Superparamagnetic iron oxide nanoparticles
multi walled carbon nanotubes
Bovine serum albumin
Biological surface adsorption index
Circular dichroism spectrometry
Fourier transformed infrared spectrometry
Nuclear magnetic resonance.
Project supported by National Health and Medical Research Council (NHMRC) project grant #616621. A. L. is an Australian Research Council - Future Fellow. A.D. acknowledges financial support from the FP7 European project NanoValid (contract no. 263147) and from the University of Salzburg Priority Program ‘Biosciences and Health’.
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