A nanoliposome delivery system to synergistically trigger TLR4 AND TLR7
© Fox et al.; licensee BioMed Central Ltd. 2014
Received: 8 January 2014
Accepted: 14 April 2014
Published: 26 April 2014
Recent reports that TLR4 and TLR7 ligands can synergistically trigger Th1 biased immune responses suggest that an adjuvant that contains both ligands would be an excellent candidate for co-administration with vaccine antigens for which heavily Th1 biased responses are desired. Ligands of each of these TLRs generally have disparate biochemical properties, however, and straightforward co-formulation may represent an obstacle.
We show here that the TLR7 ligand, imiquimod, and the TLR4 ligand, GLA, synergistically trigger responses in human whole blood. We combined these ligands in an anionic liposomal formulation where the TLR7 ligand is in the interior of the liposome and the TLR4 ligand intercalates into the lipid bilayer. The new liposomal formulations are stable for at least a year and have an attractive average particle size of around 140 nm allowing sterile filtration. The synergistic adjuvant biases away from Th2 responses, as seen by significantly reduced IL-5 and enhanced interferon gamma production upon antigen-specific stimulation of cells from immunized mice, than any of the liposomal formulations with only one TLR agonist. Qualitative alterations in antibody responses in mice demonstrate that the adjuvant enhances Th1 adaptive immune responses above any adjuvant containing only a single TLR ligand as well.
We now have a manufacturable, synergistic TLR4/TLR7 adjuvant that is made with excipients and agonists that are pharmaceutically acceptable and will have a straightforward path into human clinical trials.
KeywordsTLR4 TLR7 Synergy Adjuvant Manufacturability Liposome
Modern, effective vaccines rely on a combination of a purified antigen against which an immune response is desired and an adjuvant that triggers the innate immune system to enhance the magnitude and quality of the generated immune response . Producing new adjuvants that can direct appropriate immunity is therefore becoming key to vaccine design and groups have shown that even in the context of the same antigen different outcomes are achieved as a function of the co-administered adjuvant . Recent commercial approval of the Cervarix® vaccine that contains MPL, a defined TLR4 ligand, has added momentum to the development of a new generation of adjuvants .
The immune system has the inborn ability to recognize molecular signatures carried by microbes. These Microbe Associated Molecular Patterns (MAMPs) trigger any of an array of receptor sensors in cells to alert the organism and mobilize an appropriate immune response. MAMPs are generally molecules that are vital to the microbe, performing key functions, but are not found in the host. Examples include: lipopolysaccharides (LPS) found on the surface of gram negative bacteria , RNAs produced as part of viral replication , and flagellins that make up bacterial flagella .
The Toll-Like Receptor (TLR) family of proteins is a well characterized group of innate signaling receptors that respond to a variety of MAMPs. TLRs 1,2, and 6 hetero-dimerize and respond to lipopeptides; TLR3 binds double stranded RNAs; TLR4 signals when triggered by LPSs; TLR5 senses flagellins; TLR7 and 8 detect single stranded RNAs and TLR9 responds to DNA with CpG motifs [5, 7–12]. To augment their ability to appropriately detect pathogens, the TLRs are partitioned where the likely source of the MAMP would be found; i.e. shed LPS is picked up by lipopolysaccharide binding protein and transported to the cell membrane where TLR4 is located and the viral RNA sensors TLR3, 7, and 8 are located in endosomes inside the cell where viral RNAs would be produced and their detection required [13, 14].
TLR signaling and the resulting innate and adaptive responses can be enhanced by synergy within the TLR family  or by signals from TLR combined with those from non-TLR innate sensors [16, 17]. Within the TLR family, TLR4 and TLR7 have been targeted by agonists that are in late stage development [18, 19] or are commercial [20, 21] and the two TLR show potential for powerful synergism. This can be seen in increased magnitudes of cytokine secretion, enhanced germinal center formation, class switching and antibody diversity [22, 23]. While the synergy that is induced is dramatic, the agonists have to be co-localized and efforts to develop such a combination adjuvant that can be manufactured cost effectively at scale are still needed. Researchers in academic and company settings have started to produce adjuvants and vaccine nanoparticles that contain multiple innate ligands [22, 24–26] and standard vaccine preparations usually contain several TLR ligands since they are derived from inactivated pathogens [27, 28], but most of these attempts are proof of concept exercises that have not progressed due to the cost of manufacturing or the poor definition of the relative ligand content. We report here the development of a nanoliposome that co-localizes TLR4 and TLR7 agonists and synergistically enhances immune responses. The process used for manufacture of the combination can be scalable and commercially viable like similar liposomal formulations.
In vitro synergy of GLA and IMQ
Manufacturing of the synergistic nanoliposome
After establishing that the molecules have the potential for synergistic signaling, we set out to develop a manufacturing process that would allow them to be co-localized in the same particle. Liposomes comprise a versatile formulation platform since they can be used to bring together active molecules with different structural properties. For instance, lipidic molecules such as MPL® or GLA can be incorporated in the phospholipid bilayer whereas small, more soluble molecules may be encapsulated in the aqueous core. Furthermore, several liposome-based formulations have been approved for human use or reached advanced clinical development in existing vaccine (e.g. Epaxal®, Mosquirix) and drug (e.g. Ambisome®) products. Finally, liposomes can be manufactured at small diameter (<200 nm), permitting terminal sterile filtration and improved lymph node targeting .
The formulation of IMQ presents a particular challenge due to its insolubility in aqueous solutions at physiological pH [31, 32]. In fact, IMQ has very low solubility in most organic solvents, with the exception of fatty acids which form the basis of the approved topical cream containing IMQ called Aldara® . IMQ is soluble in acidic aqueous solutions and 0.1 M lactic acid-based formulations of IMQ are in clinical development for the treatment of bladder cancer [32–34]. Although lactic acid is a widely used pharmaceutical excipient [32, 35], it does not prevent the systemic distribution of IMQ; therefore, improving its vaccine adjuvant properties requires further formulation techniques to localize the molecule in the body after administration [32, 36]. We hypothesized that liposomes with a lactic acid core would facilitate encapsulation of soluble IMQ while enabling the bulk aqueous phase external to the liposomes to maintain a physiological buffer at close to a neutral pH, which is desirable to minimize injection pain . Moreover, liposomes should allow the intercalation of the six acyl chains of GLA into the lipid bilayer.
Liposomes were manufactured using a thin-film method wherein phospholipids and GLA were mixed with cholesterol in organic solvent followed by evaporation of the solvent under vacuum. Liposomes were rehydrated in 100 mM lactic acid containing 10 mg/ml IMQ, and sonicated in a water bath at ~60°C (above the phase transition temperatures of the lipids) for ~1.5 – 3 hrs. The liposomes were then transferred to a desalting column and the external lactic acid solution was exchanged for phosphate-buffered saline (PBS) at pH 7.2.
Liposome formulation screen for encapsulation of IMQ
Number of batches
IMQ concentration (mg/ml)
IMQ loading efficiency (%)
% Loss after 0.2-μm filtration
Particle size (Zave, nm)
0.6 ± 0.1
6 ± 1
23 ± 12
151 ± 20
0.17 ± 0.07
0.7 ± 0.2
7 ± 2
45 ± 22
73 ± 23
0.20 ± 0.01
Biophysical characterization of the liposome
Comparison of liposome properties
Number of batches
Particle size (Z-ave, nm)
GLA retention efficiency (%)
pH before buffer exchange
pH after buffer exchange
64 ± 12
0.23 ± 0.05
81 ± 5
2.54 ± 0.06
6.73 ± 0.10
160 ± 25
0.21 ± 0.10
3.48 ± 0.01
6.84 ± 0.10
141 ± 5
0.14 ± 0.02
79 ± 10
3.53 ± 0.01
6.92 ± 0.01
70 ± 25
0.22 ± 0.04
2.60 ± 0.05
6.81 ± 0.07
While IMQ loading efficiency is low (Table 1), it should be noted that the loading mechanism is passive rather than the more efficient, active loading methods based on pH or ammonium sulfate gradients , although some aqueous solubility is a prerequisite of such loading methods. By employing the mathematical model developed by Xu et al. , assuming 75 mM phospholipid concentration, a monodisperse average particle size of 160 nm with polydispersity width of ± 35.2 nm, a bilayer thickness of 4.8 nm, and an average lipid molecular area of 0.4 nm2[41, 42], the expected passive encapsulation efficiency of IMQ is predicted to be ~24%. This may be overly optimistic considering that the Z-ave size value (i.e. 160 nm for IMQ-LS) is based on scattering intensity and thus biased towards larger particles, and does not represent the true number-based size mean which may be significantly smaller, thus reducing the expected encapsulation efficiency. Nevertheless, it should be possible to improve the loading efficiency of the liposomes in the present work with further process optimization.
In earlier work, we had demonstrated that GLA intercalated into phospholipid-emulsified oil at the oil/water interface, causing a more negative zeta potential [38, 43]. The same technique applied to anionic liposomes is not as discriminatory due to the relatively high negative charge of the liposomes themselves ; nevertheless, given the insolubility of GLA and its affinity for phospholipid structures [43, 44], it is presumed that this TLR4 ligand localizes in the lipid bilayer in a similar manner to the other phospholipids. Moreover, in a previous report we employed in vitro bioactivity analysis and different order-of-mixing techniques to indicate that GLA formulated in anionic liposomes or oil-in-water emulsion is likely associated with the lipid particles rather than the bulk aqueous phase .
Ongoing work in our lab is seeking to enhance loading efficiency of liposomes containing IMQ by varying phospholipid concentration and liposome preparation techniques, such as replacing sonication with high pressure homogenization to achieve more reproducible and uniform particle size. However, even if loading efficiency remains somewhat low, the cost efficiency of IMQ-containing liposomes could still be quite favorable compared to other TLR7 ligands given that IMQ is available at approximately the same cost as phospholipid excipients (i.e. <$20/g) from generic manufacturers, which is not the case with newer imidazoquinoline-based TLR7 agonists.
Th1 responses in vivo
We report here the development of a synergistic adjuvant that is manufacturable and combines TLR4 and TLR7 ligands. We were able to develop a process by which anionic liposomes could be made that incorporate the TLR4 ligand GLA at the phospholipid bilayer interface and the TLR7 ligand IMQ in their interior. The liposome co-localizes the agonists allowing same cell activation of the external sensor TLR4 through GLA and TLR7 through imiquimod. Therefore, the same cell would experience simultaneous triggering of two innate sensors, resulting in an enhanced cytokine response.
Chemicals and reagents
Milled imiquimod (IMQ) was purchased from Chemagis (Bnei Brak, Israel); imiquimod was also obtained from Invivogen (San Diego, CA). Glucopyranosyl lipid adjuvant (GLA), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and 1,2,dipalmitoyl-3-trimethylammonium-propane (DPTAP) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL). DOPC was also purchased from Lipoid (Newark, NJ). Cholesterol, and ammonium phosphate mono- and dibasic were purchased from J.T. Baker (San Francisco, CA). Lactic acid was purchased from Sigma-Aldrich (St. Louis, MO). Phosphate buffered saline (PBS) at pH 7.2 was purchased from Life Technologies (Grand Island, NY).
Liposome formulations were manufactured by first combining phospholipids, cholesterol, and optionally, depending on the desired type of adjuvant, GLA, in chloroform:methanol or chloroform:methanol:water, which was then evaporated overnight using a Genevac EZ-2 Plus Evaporator (Stone Ridge, New York). The dried components were rehydrated in either 100 mM lactic acid with or without 10 mg/ml IMQ, and then sonicated in a VWR 75D (West Chester, PA) or Crest Powersonic CP230D (Trenton, NJ) water batch sonicator at ~60°C for 1.5 – 3 hrs, until the formulation appeared homogeneous and translucent. This formulation was kept in a heated water bath prior to transferring 2.5 ml to a separate disposable PD-10 desalting column obtained from GE Healthcare Bio-Sciences AB (Uppsala, Sweden). The columns arrived pre-packed with Sephadex G-25 medium and were primed and subsequently eluted with 3.5 ml of PBS (pH 7.2). The buffer exchange step induces a ~30% dilution of each formulation and is employed to exchange the bulk aqueous phase external to the liposomes from lactic acid to PBS, and to remove non-encapsulated IMQ. GLA-LS formulations were then mixed with GLA-IMQ-LS formulations in order to generate final formulations with different doses of IMQ.
Characterizing the formulations
Particle size of all liposomal formulations was monitored by dynamic light scattering (DLS) using the Malvern Instruments (Worcestershire, UK) Zetasizer Nano-S or Nano-ZS. Samples were prepared at 1:100 dilutions by combining 5 μl of each formulation with 500 μl of ultrapure water in a 1.5 ml polystyrene disposable cuvette. DLS measurements were then made three times on each cuvette. Formulations containing GLA were analyzed by reverse-phase high performance liquid chromatography (HPLC) with charged aerosol detection (CAD) to determine GLA concentration as previously published  except that in some cases the column employed was a Waters XBridge C18 (Milford, MA). Formulations containing IMQ were analyzed by UV–vis spectroscopy (Hitachi U-3900H, Tokyo, Japan) to confirm IMQ concentration via absorbance at 307 nm. The pH of the formulation before and after the buffer exchange was also measured. Liposome formulations containing IMQ were prepared in triplicates by combining 50 μl of sample with 950 μl of EtOH/HCl (98%/2%) into three separate disposable UV-cuvettes. IMQ concentration was extrapolated from a linear 5-point standard curve. pH was measured using a Mettler Toledo (Columbus, Ohio) MP225 pH meter and an Orion Ross semi-micro 8103BN pH probe obtained from Thermo Scientific (Waltham, MA). A 3-point calibration was performed prior to measurement with pH 4.00, 7.00, and 10.00 standard buffers. To determine whether IMQ was encapsulated in the liposomes, ultracentrifuged samples were prepared by transferring 200 μl of the liposome formulation into a 1.5 ml capacity ultracentrifuge tube and centrifuging at 160,000 × g at 4°C for three 3-hour cycles using an Optima™ MAX-XP Beckman-Coulter Ultracentrifuge (Indianapolis, IN). The supernatant was removed after each of the first two cycles and the pellet at the bottom of the tube was washed, each time with 1 ml of PBS with gentle mixing, followed by another 3 hours of ultracentrifugation at 160,000 × g at 4˚C, and subsequent removal of the supernatant. The liposome pellet was then lysed with 1.05 ml ethanol/concentrated hydrochloric acid (98%/2%), sonicated for ~5 mins, and diluted 1:20 in the same solvent mixture for spectrophotometric analysis.
In-vitro stimulation using a whole blood assay (WBA)
After obtaining informed consent, heparinized whole blood was collected from healthy volunteers and 180 μl plated directly into 96-well round-bottom tissue culture plates. 20 μl of each formulation with the various innate stimuli were then added giving final well volumes of 200 μl. Each stimulation condition was conducted in duplicate. IMQ from Invivogen was suspended in dH2O to a concentration of 5 mg/ml. GLA-AF was manufactured as described in Orr et al. at a concentration of 1 mg/ml. IMQ and GLA were diluted in PBS prior to addition, either separately and in combination, to blood, which was then incubated at 37°C, 5% CO2 for 24 h. After incubation, 100 μl of the plasma supernatant was carefully extracted and cytokine content measured by ELISA: Mip-1β (R & D Systems, Minneapolis, MN); IL-8, IL-12p70, and IFNγ (eBioscience, San Diego, CA).
Mice and immunizations
Plasmodium berghei circumsporozoite protein (PbCSP) was expressed and purified from E. coli using the codon-harmonized construct kindly provided by Dr. Evelina Angov from the Walter Reed Army Institute of Research. All animal protocols were approved by the IDRI institutional animal care and use committee. Female C57BL/6 mice were purchased from Charles River Laboratories (Wilmington, MA) and maintained in specific pathogen-free conditions. Mice, 6–8 weeks of age, were immunized intramuscularly three times in two-week intervals by injection at the base of the tail. For immunization, recombinant protein was formulated with adjuvant to provide a total of 2 μg protein/injection with various doses of the adjuvant in a total volume of 0.1 ml. The adjuvant doses corresponded to 20 μg IMQ and 5 μg GLA for both the single and combined adjuvants.
Blood was collected from the retro-orbital sinus two weeks after the second immunization and sera prepared. Sera were stored at 4°C until antigen-specific antibody responses were analyzed by ELISA. Briefly, ELISA plates (Nunc, Rochester, NY) were coated with 1 μg/ml antigen in 0.1 M bicarbonate buffer and blocked with 0.1% BSA-PBS. Following washes in PBS/Tween, serially diluted serum samples were added. After incubation and further washes, either anti-mouse IgG-HRP, anti-mouse-IgG2c-HRP or anti-mouse IgG1-HRP were added (all Southern Biotech, Birmingham, AL). After incubation and washing, ABTS-H2O2 (Kirkegaard and Perry Laboratories, Gaithersburg, MD) was added to the plates to reveal any reactions, which were stopped by the addition of 0.1 N H2SO4. Plates were analyzed at 405 nm (ELX808, Bio-Tek Instruments Inc, Winooski, VT). Midpoint titers were determined as EC50 values from weighted curve fits using the GraphPad Prism package V 6.03.
Cell preparations and antigen stimulation assays
Six weeks after the final immunization, spleens were removed and single cell suspensions prepared. Mononuclear cells were enumerated using a ViaCount assay with a PCA system (Guava Technologies, Hayward, CA). To determine overall cytokine production, spleen cells were cultured at 2 × 105 cells per well in duplicate in a 96-well plate (Corning Incorporated, Corning, NY) in RPMI-1640 supplemented with 10% heat-inactivated FCS and 50,000 Units penicillin/streptomycin (Invitrogen), in the presence of 10 μg/ml protein. Culture supernatants were harvested after 4 days and IFNγ/IL-5 content determined by ELISA, according to the manufacturer’s instructions (eBioscience, San Diego, CA).
To determine the number of cells producing each cytokine, multiScreen 96-well filtration plates (Millipore) were coated with rat anti-mouse IL-5 or rat anti-mouse IFNγ capture antibody (both eBioscience) and incubated overnight at 4˚C. Plates were washed with PBS, blocked with RPMI 1640 and 10% FBS for at least 1 h at room temperature, and washed again. Spleen cells were then added at 2 × 105 cells/well and stimulated with media or antigen (10 μg/ml) for 48 h at 37˚C. The plates were then washed with 0.1% PBS–Tween 20 and incubated overnight with a biotin-conjugated rat anti-mouse IL-5 or IFNγ secondary antibody (eBioscience) diluted in 0.1% PBS–Tween 20/0.5% BSA. The filters were developed using the VectaStain ABC avidin peroxidase conjugate and Vectastain AEC substrate kits (Vector Laboratories, Burlingame, CA) according to the manufacturer’s protocol. The reaction was stopped by washing the plates with deionized water. Plates were dried in the dark, and spots were counted on an automated ELISPOT reader (C.T.L. Series 3A Analyzer; Cellular Technology Ltd., Shaker Heights, OH) and analyzed with ImmunoSpot software (Cellular Technology Ltd).
Five mice were immunized for each treatment with two subsamples taken per mouse. These subsamples were handled by treating mice within each treatment as a nested random effect to allow the separation of the variability between mice from the variability within each mouse. In order to test Th1 and Th2 responses in vivo using ELISA determination of levels of secreted cytokines and ELISPOT enumerations of the number of specific cytokine screening cells for both IL-5 and IFNγ secretions, four one-way ANOVA tests were used. Each test was used to compare mean differences between mice immunized with either antigen, the liposomal carrier, IMQ in liposomes, GLA in liposomes, or the combination adjuvant. Tukey’s procedure was used to test pairwise comparisons between treatments if significant treatment effects were found. Consideration of the variance estimates for the nested factor indicated that, in all cases, the majority of the variation in the data was due to variation between mice rather than variation among the subsamples taken from individual mice. All hypothesis testing was done at the 95% level.
3-O-desacyl-4′-monophosphoryl lipid A
Macrophage inflammatory protein 1-beta
Glucopyranosyl lipid adjuvant
We thank Dr. Evelina Angov from the Walter Reed Army Institute of Research for providing the antigen used in these experiments, PbCSP. This research was supported with funding from the Bill and Melinda Gates Foundation, under grants 42387 and OPP1055855.
- Carter D, Reed SG: Role of adjuvants in modeling the immune response. Curr Opin HIV AIDS. 2010, 5: 409-413. 410.1097/COH.1090b1013e32833d32832cdbView ArticleGoogle Scholar
- Ansong D, Asante KP, Vekemans J, Owusu SK, Owusu R, Brobby NAW, Dosoo D, Osei-Akoto A, Osei-Kwakye K, Asafo-Adjei E, Boahen KO, Sylverken J, Adjei G, Sambian D, Apanga S, Kayan K, Janssens MH, Lievens MJJ, Olivier AC, Jongert E, Dubois P, Savarese BM, Cohen J, Antwi S, Greenwood BM, Evans JA, Agbenyega T, Moris PJ, Owusu-Agyei S: T cell responses to the RTS,S/AS01<sub>E</sub> and RTS,S/AS02<sub>D</sub> malaria candidate vaccines administered according to different schedules to Ghanaian children. PLoS One. 2011, 6: e18891-View ArticleGoogle Scholar
- Harper DM, Vierthaler SL: Next generation cancer protection: the bivalent HPV vaccine for females. ISRN Obstet Gynecol. 2011, 2011: 457204-View ArticleGoogle Scholar
- Ray A, Cot M, Puzo G, Gilleron M, Nigou J: Bacterial cell wall macroamphiphiles: pathogen-/microbe-associated molecular patterns detected by mammalian innate immune system. Biochimie. 2013, 95: 33-42.View ArticleGoogle Scholar
- Meylan E, Tschopp J: Toll-like receptors and RNA helicases: two parallel ways to trigger antiviral responses. Mol Cell. 2006, 22: 561-569.View ArticleGoogle Scholar
- Hayashi F, Smith KD, Ozinsky A, Hawn TR, Yi EC, Goodlett DR, Eng JK, Akira S, Underhill DM, Aderem A: The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 2001, 410: 1099-1103.View ArticleGoogle Scholar
- Song DH, Lee JO: Sensing of microbial molecular patterns by Toll-like receptors. Immunol Rev. 2012, 250: 216-229.View ArticleGoogle Scholar
- Johnson TR, Rao S, Seder RA, Chen M, Graham BS: TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity. Vaccine. 2009, 27: 3045-3052.View ArticleGoogle Scholar
- Park BS, Song DH, Kim HM, Choi BS, Lee H, Lee JO: The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex. Nature. 2009, 458: 1191-1195.View ArticleGoogle Scholar
- Lu YC, Yeh WC, Ohashi PS: LPS/TLR4 signal transduction pathway. Cytokine. 2008, 42: 145-151.View ArticleGoogle Scholar
- Gorski KS, Waller EL, Bjornton-Severson J, Hanten JA, Riter CL, Kieper WC, Gorden KB, Miller JS, Vasilakos JP, Tomai MA, Alkan SS: Distinct indirect pathways govern human NK-cell activation by TLR-7 and TLR-8 agonists. Int Immunol. 2006, 18: 1115-1126.View ArticleGoogle Scholar
- Malhotra D, Relhan V, Reddy BS, Bamezai R: TLR2 Arg677Trp polymorphism in leprosy: revisited. Hum Genet. 2005, 116: 413-415.View ArticleGoogle Scholar
- Kawai T, Akira S: TLR signaling. Semin Immunol. 2007, 19: 24-32.View ArticleGoogle Scholar
- Miggin SM, O’Neill LA: New insights into the regulation of TLR signaling. J Leukoc Biol. 2006, 80: 220-226.View ArticleGoogle Scholar
- Raman VS, Bhatia A, Picone A, Whittle J, Bailor HR, O’Donnell J, Pattabhi S, Guderian JA, Mohamath R, Duthie MS, Reed SG: Applying TLR synergy in immunotherapy: implications in cutaneous leishmaniasis. J Immunol. 2010, 185: 1701-1710.View ArticleGoogle Scholar
- Brosbol-Ravnborg A, Bundgaard B, Hollsberg P: Synergy between vitamin D3 and Toll-like receptor agonists regulates human dendritic cell response during maturation. Clin Dev Immunol. 2013, 2013: 807971-View ArticleGoogle Scholar
- Pone EJ, Zhang J, Mai T, White CA, Li G, Sakakura JK, Patel PJ, Al-Qahtani A, Zan H, Xu Z, Casali P: BCR-signalling synergizes with TLR-signalling for induction of AID and immunoglobulin class-switching through the non-canonical NF-kappaB pathway. Nat Commun. 2012, 3: 767-View ArticleGoogle Scholar
- Coler RN, Bertholet S, Moutaftsi M, Guderian JA, Windish HP, Baldwin SL, Laughlin EM, Duthie MS, Fox CB, Carter D, Friede M, Vedvick TS, Reed SG: Development and characterization of synthetic glucopyranosyl lipid adjuvant system as a vaccine adjuvant. PLoS One. 2011, 6: e16333-View ArticleGoogle Scholar
- Zhang WW, Matlashewski G: Immunization with a Toll-like receptor 7 and/or 8 agonist vaccine adjuvant increases protective immunity against Leishmania major in BALB/c mice. Infect Immun. 2008, 76: 3777-3783.View ArticleGoogle Scholar
- Didierlaurent AM, Morel S, Lockman L, Giannini SL, Bisteau M, Carlsen H, Kielland A, Vosters O, Vanderheyde N, Schiavetti F, Larocque D, Van Mechelen M, Garcon N: AS04, an aluminum salt- and TLR4 agonist-based adjuvant system, induces a transient localized innate immune response leading to enhanced adaptive immunity. J Immunol. 2009, 183: 6186-6197.View ArticleGoogle Scholar
- El-On J, Bazarsky E, Sneir R: Leishmania major: in vitro and in vivo anti-leishmanial activity of paromomycin ointment (Leshcutan) combined with the immunomodulator imiquimod. Exp Parasitol. 2007, 116: 156-162.View ArticleGoogle Scholar
- Kasturi SP, Skountzou I, Albrecht RA, Koutsonanos D, Hua T, Nakaya HI, Ravindran R, Stewart S, Alam M, Kwissa M, Villinger F, Murthy N, Steel J, Jacob J, Hogan RJ, Garcia-Sastre A, Compans R, Pulendran B: Programming the magnitude and persistence of antibody responses with innate immunity. Nature. 2011, 470: 543-547.View ArticleGoogle Scholar
- Wiley SR, Raman VS, Desbien A, Bailor HR, Bhardwaj R, Shakri AR, Reed SG, Chitnis CE, Carter D: Targeting TLRs expands the antibody repertoire in response to a malaria vaccine. Sci Transl Med. 2011, 3: 93ra69-View ArticleGoogle Scholar
- Ali OA, Verbeke C, Johnson C, Sands W, Lewin SA, White D, Doherty E, Dranoff G, Mooney DJ: Identification of immune factors regulating anti-tumor immunity using polymeric vaccines with multiple adjuvants. Cancer Res. 2014, 74: 1670-1681.View ArticleGoogle Scholar
- Shima F, Uto T, Akagi T, Akashi M: Synergistic stimulation of antigen presenting cells via TLR by combining CpG ODN and poly(gamma-glutamic acid)-based nanoparticles as vaccine adjuvants. Bioconjug Chem. 2013, 24: 926-933.View ArticleGoogle Scholar
- Yang M, Flavin K, Kopf I, Radics G, Hearnden CH, McManus GJ, Moran B, Villalta-Cerdas A, Echegoyen LA, Giordani S, Lavelle EC: Functionalization of carbon nanoparticles modulates inflammatory cell recruitment and NLRP3 inflammasome activation. Small. 2013, 9: 4194-4206.View ArticleGoogle Scholar
- Geeraedts F, Goutagny N, Hornung V, Severa M, de Haan A, Pool J, Wilschut J, Fitzgerald KA, Huckriede A: Superior immunogenicity of inactivated whole virus H5N1 influenza vaccine is primarily controlled by Toll-like receptor signalling. PLoS Pathog. 2008, 4: e1000138-View ArticleGoogle Scholar
- Schreibelt G, Benitez-Ribas D, Schuurhuis D, Lambeck AJ, van Hout-Kuijer M, Schaft N, Punt CJ, Figdor CG, Adema GJ, de Vries IJ: Commonly used prophylactic vaccines as an alternative for synthetically produced TLR ligands to mature monocyte-derived dendritic cells. Blood. 2010, 116: 564-574.View ArticleGoogle Scholar
- Invivogen: Imiquimod - R837 technical data sheet. 2013Google Scholar
- Bachmann MF, Jennings GT: Vaccine delivery: a matter of size, geometry, kinetics and molecular patterns. Nat Rev Immunol. 2010, 10: 787-796.View ArticleGoogle Scholar
- Chollet JL, Jozwiakowski MJ, Phares KR, Reiter MJ, Roddy PJ, Schultz HJ, Ta QV, Tomai MA: Development of a topically active imiquimod formulation. Pharm Dev Technol. 1999, 4: 35-43.View ArticleGoogle Scholar
- Hayashi T, Crain B, Corr M, Chan M, Cottam HB, Maj R, Barberis A, Leoni L, Carson DA: Intravesical Toll-like receptor 7 agonist R-837: optimization of its formulation in an orthotopic mouse model of bladder cancer. Int J Urol. 2010, 17: 483-490.View ArticleGoogle Scholar
- Falke J, Lammers RJ, Arentsen HC, Ravic M, Pozzi R, Cornel EB, Vergunst H, de Reijke TM, Witjes JA: Results of a phase 1 dose escalation study of intravesical TMX-101 in patients with nonmuscle invasive bladder cancer. J Urol. 2013, 189: 2077-2082.View ArticleGoogle Scholar
- Arentsen HC, de Kaa CA H-V, Jansen CF, Maj R, Leoni LM, Oosterwijk E, Witjes JA: Pharmacokinetics and toxicity of intravesical TMX-101: a preclinical study in pigs. BJU Int. 2011, 108: 1210-1214.View ArticleGoogle Scholar
- Food and Drug Administration: Inactive ingredient search for approved drug products. U.S. Food and Drug Administration
- Alkan SS: Skills, knowledge, and translational technologies leading to novel vaccines. Development of novel vaccines: skills, knowledge and translational technologies. Edited by: Von Gabain A, Klade C. New York: Springer, 2006, 1-2.Google Scholar
- Fransson J, Espander-Jansson A: Local tolerance of subcutaneous injections. J Pharm Pharmacol. 1996, 48: 1012-1015.View ArticleGoogle Scholar
- Orr MT, Fox CB, Baldwin SL, Sivananthan SJ, Lucas E, Lin S, Phan T, Moon JJ, Vedvick TS, Reed SG, Coler RN: Adjuvant formulation structure and composition are critical for the development of an effective vaccine against tuberculosis. J Control Release. 2013, 172: 190-200.View ArticleGoogle Scholar
- Barenholz Y: Doxil® — The first FDA-approved nano-drug: lessons learned. J Control Release. 2012, 160: 117-134.View ArticleGoogle Scholar
- Fenske DB, Maurer N, Cullis PR: Encapsulation of weakly-basic drugs, antisense oligonucleotides, and plasmid DNA within large unilamellar vesicles for drug delivery applications. Liposomes: a practical approach. 2nd edition. Edited by: Torchilin VP, Weissig V. New York: Oxford University Press, 2003.Google Scholar
- Xu X, Khan MA, Burgess DJ: Predicting hydrophilic drug encapsulation inside unilamellar liposomes. Int J Pharm. 2012, 423: 410-418.View ArticleGoogle Scholar
- Edholm O, Nagle JF: Areas of molecules in membranes consisting of mixtures. Biophys J. 2005, 89: 1827-1832.View ArticleGoogle Scholar
- Anderson RC, Fox CB, Dutill TS, Shaverdian N, Evers TL, Poshusta GR, Chesko J, Coler RN, Friede M, Reed SG, Vedvick TS: Physicochemical characterization and biological activity of synthetic TLR4 agonist formulations. Coll Surf B: Biointerfaces. 2010, 75: 123-132.View ArticleGoogle Scholar
- Fox CB: Characterization of TLR4 agonist effects on Alhydrogel sedimentation: a novel application of laser scattering optical profiling. J Pharm Sci. 2012, 101: 4357-4364.View ArticleGoogle Scholar
- Misquith A, Fung HWM, Dowling QM, Guderian JA, Vedvick TS, Fox CB: In vitro evaluation of TLR4 agonist activity: formulation effects. Colloids Surf B: Biointerfaces. 2014, 113: 312-319.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.