Silica nanoparticles for the layer-by-layer assembly of fully electro-active cytochrome c multilayers
© Feifel and Lisdat; licensee BioMed Central Ltd. 2011
Received: 12 October 2011
Accepted: 30 December 2011
Published: 30 December 2011
For bioanalytical systems sensitivity and biomolecule activity are critical issues. The immobilization of proteins into multilayer systems by the layer-by-layer deposition has become one of the favorite methods with this respect. Moreover, the combination of nanoparticles with biomolecules on electrodes is a matter of particular interest since several examples with high activities and direct electron transfer have been found. Our study describes the investigation on silica nanoparticles and the redox protein cytochrome c for the construction of electro-active multilayer architectures, and the electron transfer within such systems. The novelty of this work is the construction of such artificial architectures with a non-conducting building block. Furthermore a detailed study of the size influence of silica nanoparticles is performed with regard to formation and electrochemical behavior of these systems.
We report on interprotein electron transfer (IET) reaction cascades of cytochrome c (cyt c) immobilized by the use of modified silica nanoparticles (SiNPs) to act as an artificial matrix. The layer-by-layer deposition technique has been used for the formation of silica particles/cytochrome c multilayer assemblies on electrodes. The silica particles are characterized by dynamic light scattering (DLS), Fourier transformed infrared spectroscopy (FT-IR), Zeta-potential and transmission electron microscopy (TEM). The modified particles have been studied with respect to act as an artificial network for cytochrome c and to allow efficient interprotein electron transfer reactions. We demonstrate that it is possible to form electro-active assemblies with these non-conducting particles. The electrochemical response is increasing linearly with the number of layers deposited, reaching a cyt c surface concentration of about 80 pmol/cm2 with a 5 layer architecture. The interprotein electron transfer through the layer system and the influence of particle size are discussed.
This study demonstrates the ability to construct fully electro-active cyt c multilayer assemblies by using carboxy-modified silica nanoparticles. Thus it can be shown that functional, artificial systems can be build up following natural examples of protein arrangements. The absence of any conductive properties in the second building block clearly demonstrates that mechanisms for electron transfer through such protein multilayer assemblies is based on interprotein electron exchange, rather than on wiring of the protein to the electrode.
The construction strategy of this multilayer system provides a new controllable route to immobilize proteins in multiple layers featuring direct electrochemistry without mediating shuttle molecules and controlling the electro-active amount by the number of deposition steps.
Silica particles at the nano- and microscale are widely used in various areas of science [1, 2]. Their universalism is due to the ease of preparation and possibility of controlling size, a high surface-to-volume ratio, and the biocompatibility of silica. Accordingly, several synthetic approaches for the synthesis of silica nanoparticles (SiNPs) are available [3–5]. Different surface modification protocols have been developed in order to immobilize various biomolecules such as enzymes, proteins, and DNA [6–8]. As a stable solid support for such molecules or biomolecular conjugates they have opened the door to applications in sensors , drug delivery system , and smart materials . For bioanalytical systems sensitivity and biomolecule activity are very critical issues. SiNPs can provide an artificial matrix and thus keeping and enhancing the bioactivity. Various approaches have been developed for increasing the sensitivity of sensors [12–18]. Particular advantage is the defined increase in surface concentrations of the recognition element. The immobilization of proteins into multilayer systems by the layer-by-layer deposition has become one of the favorite methods [19–24]. Moreover, the combination of nanoparticles with biomolecules on electrodes is a matter of particular interest since several examples with direct electron transfer have been found [25–29]. By the use of the redox protein cyt c and the polyelectrolyte polyaniline sulfonic acid (PASA) fully electro-active multilayers have been constructed on electrodes. Raising the number of layers a continuous increase of the voltammetric cyt c signal has been achieved with this system and can be used for the detection of superoxide radicals with enhanced sensitivity [30, 31]. Such multilayer assemblies have recently been shown to enable incorporation of enzymes and establish communication to the electrode, thus allowing the construction of different analytical signal chains [24, 32, 33]. SiNPs have already been used in biosensors since the high surface area of nano-sized silica particles can increase the surface molecule loading and thus lead to a higher performance of the biosensor [25, 34–36].
In this study we want to show that silica nanoparticles can be used as building blocks in layered architecture of proteins on electrodes. It can be expected that the particle size and the surface charge of the used silica nanoparticles play a key role in modulating the properties of such multilayer architectures.
We propose that multilayer assemblies with the redox protein cyt c and different-sized surface modified SiO2 particles, ranging from 5 - 60 nm can lead to novel architectures in which the cyt c molecules can exhibit direct electrochemistry. Thereby we combine the layer-by-layer technique and modified SiNPs for construction of fully electro-active cyt c multilayer electrodes. The conditions of assembly formation and the stability are determined by quarz crystal microbalance (QCM). The electrochemical properties of the multilayer architectures are analyzed by cyclic voltammetry (CV). Special focus is on the size influence of the particles and the mediator-free electron transfer ability of the multilayer assembly.
Results and Discussion
We select cytochrome c and silica nanoparticles for this study on the basis of the following considerations. First, silica nanoparticles can be synthesized and functionalized in a variety of sizes, and characterized by a narrow particle size distribution. Second, silica is a dielectric material, which does not absorb light or conduct electrons. As an inert host, it may enable the assembly of biological materials (e.g. cyt c) while keeping its natural function.
Finally, the pI values of carboxy-modified silica colloids and cytochrome c are acidic and basic respectively [37, 38], and therefore, sufficient Coulomb interaction can be expected between silica and the opposite charged cytochrome c.
Synthesis and characterization of SiNPs
Modified SiO2-NH2 spheres
Modified SiO2-CO2H spheres
Adsorption characteristics of SiNPs and cyt c
Multilayer assembly of SiNPs and cyt c
On the basis of the binding experiments the construction of SiNP/cyt c -multilayers has been performed first on QCM gold chips to study the impact of the particle size on the binding performance by the use of different-sized SiNPs (5, 8, 10, 15, 20, 40, 60 nm, Figure 5). If the particle size exceeds 20 nm the binding and accordingly the formation of the multilayer assembly (cyt c/SiNP) can hardly be detected. Nevertheless, a recharging at the surface can be concluded since a further adsorption of SiNPs after the cyt c incubation is feasible. This may be attributable to a partial displacement of the larger SiNPs (20 and 40 nm) during the adsorption of cyt c. For the smaller sized SiNPs (Ø = 5 - 15 nm) however, successful assembly formations have been detected (Figure 5). The adsorbed amount of cyt c increases rather linearly with growing number of layers. For comparison polyelectrolyte-based protein assemblies with polyaniline sulfonic acid or DNA have shown an exponential layer growth. The reason for this difference can be attributed to a different structure of SiNP/cyt c -multilayers. The SiNP-layers seem to be more ordered than polyelectrolyte-based layers, because of a rather high homogeneity of the SiNPs and the absence of layer interpenetration which is discussed as one reason for exponential layer growth [39, 40].
Electrochemical characteristics of the multilayer assemblies (SiNPs/cyt c)
An electron transfer rate constant ks for all cyt c molecules in the multilayer assembly cannot be given since the reaction rate decreases with increasing distance to the electrode. This circumstance can be seen by experiments with increasing scan rate; thereby a decrease in the electro-active cyt c amount is found. At higher scan rates cyt c molecules in the outer shells cannot take part in the redox conversion since the rate of potential change exceeds the rate of electron transfer through the assembly to the electrode. Therefore only a ks-range of 27-75 s-1 can be given. Here the latter value reflects ks of cyt c immobilized in the first monolayer directly on the MU/MUA promoter, and the first value reflects ks of cyt c in a 5-layered cyt c/SiNPs (5 nm) assembly.
To gain a further impression on the reaction rate between the cyt c molecules within the assembly the self-exchange rate kex for the immobilized cytochrome c is estimated. Therefore, the Dahms-Ruff equation has been used, assuming an intermolecular electron hopping process between the cyt c molecules in the multilayer film:
The effective diffusion coefficient Deffective is calculated according to the Randles-Sevcik equation by evaluating the peak currents at small scan rates. For the cytochrome c (horse heart) in the multilayer, a Deffective of 4.96 × 10-12 cm2 s-1 is calculated. The approximate distance between the adjacent redox centers δ is estimated to be 2.6 nm and a reasonable cyt c concentration of 23 mM is used. With these values, a self-exchange rate constant of 1.99 × 104 M-1 s-1 for the cytochrome c in the multilayer is calculated. The literature values for kex of horse heart cyt c in solution are in the range of 102 - 105 M-1 s-1 depending on the ionic strength. The rather high kex for the cyt c in the multilayer assembly reflects the small values found for peak separation, and therefore is a further indication of a rather fast electron transfer within the cyt c multilayer system. In the SiNPs-based multilayer assemblies the formal potential is comparable to cyt c immobilized as a monolayer, e.g. on MU/MUA  and can be determined to be -24 ± 5 mV vs. Ag/AgCl.
In other multilayer systems using PASA or DNA as building blocks the electron transfer has been suggested to occur via protein-protein electron exchange [30, 31]. But till this day no definite proof can be given that the polyelectrolyte or DNA are not acting as conducting mediators between the cyt c molecules within the assemblies. Based on the current work using non-conductive SiNPs it becomes clear that artificial multilayer architectures can be prepared with the interprotein electron exchange as the dominating mechanism.
This observation reflects almost the results of the adsorption experiments by QCM. This means that the low electrochemical response for layer structures with larger particles is mainly caused by a less efficient layer formation process. The observations during this work anticipate that the nanoparticle size strongly influences cyt c-silica nanoparticle interactions.
In conclusion a certain balance on the interactive forces seems to be necessary for a stable layer formation on the one side and rotational flexibility of cyt c within the system for efficient electron exchange on the other side. This works best when both components of the assembly are of comparable size.
This study demonstrates the ability to construct fully electro-active cyt c multilayer assemblies by using carboxy-modified silica nanoparticles of small diameter (5 - 8 nm) as second building block. The absence of any conductive properties in the second building block clearly demonstrates that mechanisms for electron transfer through such protein multilayer assemblies is based on interprotein electron exchange, rather than on wiring of the protein to the electrode. Furthermore it is demonstrated that polymer-free cyt c multilayer assemblies can be built up.
Prepared on gold electrodes these multilayer architectures give a well defined, quasi-reversible response of cyt c. The formal potential of cyt c is not significantly influenced by the immobilization and is around -24 ± 5 mV vs. Ag/AgCl. However the peak separation is rather small. This is indicative for a fast and efficient electron transfer through the layered system. The peak width at half peak height for the multilayer system (132 ± 6 mV) is almost the same as for a cyt c monolayer (130 ± 5 mV). This shows that the cyt c molecules within the assembly are present in equal states, and thereby the protein molecules are expected to be bound in a rather similar microenvironment within the SiNPs matrix.
SiNP-based cyt c assemblies exhibit a linear increase of the cyt c amount with the number of adsorption steps as shown by the voltammetric response. For a 5-layer assembly an electro-active surface concentration of 80 ± 10 pmol/cm2 can be found. A significant increase in the amount of protein with the number of layers is also verified by QCM. Furthermore it can be demonstrated that the cyt c/SiNP assemblies are stable without any thermal treatment at low ion strength at neutral pH.
The construction strategy of this multilayer system provides a new controllable route to immobilize proteins in multiple layers featuring direct electrochemistry without mediating shuttle molecules. Our system offers new possibilities particularly for biosensing. For this purpose, the use of a multilayer sensor with higher amounts of electro-active redox proteins (e.g. cyt c) can be expected to yield a significantly enhanced sensitivity.
The efficient reproducible DET and IET through several layers also provide an opportunity to simulate biological electron transport systems based on redox proteins. Hence the approach may be applicable to the construction of a new generation of signal chains for bioelectronic functionalities.
Further studies are thus directed to generalize the effects observed in this work to other proteins and biological molecules.
Materials and methods
Description of Chemicals
Mercaptoundecanoic acid (MUA), 11-mercapto-1-undecanol (MU), horse heart cytochrome c (cyt c), ammonium hydroxide (99.99%), tetraethyl orthosilicate (99.99%), ethanol absolute, (3-aminopropyl)-triethoxysilane, succinic acid anhydride, toluene anhydrous (99.8%) are purchased from Sigma-Aldrich (Steinheim, Germany), tetrahydrofuran spectranal is purchased from Riedel-de Haen (Seelze, Germany), di-potassium hydrogen phosphate, potassium di-hydrogen phosphate are provided by MERCK (Darmstadt, Germany), and gold-wire electrodes by Goodfellow (Bad Nauheim, Germany), Goldchips for QCM are delivered by QSense (Froelunda, Sweden). Two different buffers have been used during these investigations: 5 mM potassium phosphate buffer pH 7 and 1 mM potassium phosphate buffer pH 7.
Synthesis of monodispersed SiNPs
Mono-dispersed spherical silica nanoparticles were synthesized following the Stöber-Fink-Bohn method starting from tetraethyl orthosilicate (TEOS), water, ammonia, and absolute ethanol, as precursor alkoxide, hydrolyzing agent, catalyst and solvent, respectively . The overall experimental procedure is shown in Figure 1. Two mother solutions were prepared: one containing ammonia-water, the other containing TEOS-ethanol. The same volumes of the two solutions were always mixed in a thermostatically controlled water bath (45 ± 1°C). A micro feed pump Harvard Apparatus (Model 11 Plus) with a constant flow rate (5.0 mL/min) fed the starting solution A (TEOS, ethanol) into the reactor to solution B (ammonia, water, ethanol) at 45°C and vigorously stirring, thereafter the mixture prepared was agitated for 1 h to 5 d, dependent on the particle size to be synthesized. The SiO2 dispersion was transferred out of the reactor and centrifuged (at 4.000-14.000 rpm for 1 h). The precipitate was washed with ethanol by repeated centrifugation (at 4.000 -14.000 rpm for 1 h) and dried at 70°C for 12 h.
Preparation of γ-aminopropyl modified silica nanoparticles SiO2-NH2
The amino groups modified SiO2 particles (SiO2-NH2), were prepared by the self-assembly of APTES onto the surfaces of SiO2 particles adapted from literature . First 1.0 g of SiO2 particles was charged into a 25 mL three-necked round bottom flask containing 10 mL of dry toluene, and then the suspension was dispersed with ultrasonication for 30 min. Secondly the reaction flask was equipped with an N2 inlet, a thermometer, and a Graham condenser. Then, 2.5 mL of APTES was added quickly and the suspension was refluxed at 110°C for 12 h under N2 atmosphere and magnetic stirring. After the reaction was finished, the suspension was centrifuged at 4.500 - 14.000 rpm for 1 h and the precipitate was collected. Finally, the precipitate was redispersed into 25 mL of dry toluene with ultrasonication for 20 min and then centrifuged again. Next, the precipitate was dispersed into anhydrous ethanol with ultrasonication and centrifuged once again. The operation of dispersion and centrifugation was repeated for three cycles, and the resulting precipitate, SiO2-NH2 was dried under vacuum at 40°C for 24 h.
Introduction of the carboxyl groups onto SiO2-NH2 particle surface
After the amino groups were grafted onto the SiO2 particle surface, they need to be coupled with succinc acid anhydride to introduce the carboxyl groups onto the surface. The coupling procedure was adapted from literature . Briefly, SiO2-NH2 particles (1.0 g) were dissolved in anhydrous THF (50 mL) and then the suspension was dispersed with ultrasonication for 30 min. Then succinc acid anhydride (3.5 g) was added in two portions to the reaction suspension at 0°C and stirred for 2 h. Afterwards the reaction mixture was stirred at room temperature for another 24 h. The remaining succinc acid anhydride was hydrolyzed by addition of water. The resulting product (SiO2-COOH) was dispersed by ultrasonication for 15 minutes and centrifuged at 4,000 - 14,000 rpm for 1 h. Next, the precipitate was redispersed in anhydrous THF and centrifuged again. Finally, the precipitate was dispersed into water and centrifuged for another 30 min at 4,000 rpm, and the resulting precipitate, SiO2-COOH, was dried under vacuum at 40°C for 24 h.
Characterization of the prepared silica particles
The synthesized SiNPs were characterized by dynamic light scattering analysis (DLS). DLS was used to monitor the change in hydrodynamic radius (particle size) and aggregates. Measurements were carried out on a Beckman Coulter Delsa Nano C Particle Analyzer (Krefeld, Germany) working at a fixed angle of 90° in ethanol or water to obtain the number-average diameters of the particles. Each analysis was repeated three times to give the average particle size.
Zeta-potential of the SiNPs was measured with a Beckman Coulter Delsa Nano C Zeta Potential Analyzer (Krefeld, Germany) and the measurement was repeated three times, and the average of them was reported as the final result.
FT-IR analysis for monitoring the surface modification on SiNPs was measured by Fourier transform infrared spectroscopy (FT-IR) with a Varian 680-IR FT-IR spectrometer (Varian, Australia), ATR (Diamond), resolution 8 cm-1, scans 16, in the range of 400 - 4.000 cm-1.
TEM measurements were applied to characterize the morphology and size of the different SiNPs, with a FEI Tecnai G2 20 S-TWIN transmission electron microscope, 200 kV operation voltage, resolution 0.24 nm, EDAX EDX-system with a Si(Li)-detector, detection limit start at Bor (Z = 5).
Fabrication of mono- and multilayer assemblies with SiO2-NPs
Gold-wire electrodes are cleaned by 2 times incubation in piranha solution (3:1 H2SO4/H2O2) for 10 min. The electrodes are washed with Millipore water after the cleaning steps. For the construction of multilayers the electrodes are modified by incubation for 48 h in 5 mM 3:1 solution of mercaptoundecanol/mercaptoundecanoic acid. The cyt c monolayers are prepared by incubation of the electrodes in 20 μM cyt c in KPP 5 mM pH 7 for 2 h . The assembly of SiNPs/cyt c multilayers has been performed by alternating incubations of the cyt c monolayer electrode in 20 μM cyt c and SiNPs (0.3 - 5.0 mg/ml) for 10 min per step . Each of the 10 minute long adsorption steps was followed by rinsing the electrodes in 5 mM KPP pH 7. The procedure was repeated until the desired number of layers was reached.
A Q-Sense-D E4 piezoelectric instrument (QSense, Vaestra Froelunda, Sweden) was used for the quarz crystal microbalance measurements. A clean gold covered quarz sensor chip (5 MHz, QSense, Vaestra Froelunda, Sweden) was incubated in ethanol solution containing 5 mM MUA/MU (1:3) for 24 h, then rinsed with water and mounted into the QCM flow system. The solutions containing SiNPs and cyt c of above given concentrations were successively pumped through the cell for 10 minutes with 5 minutes of buffer flow in between, at a flow rate of 25 μL/min. We estimated the mass increase [Δm (ng)] from QCM frequency shift [Δf (Hz)] of fixed films by using the Sauerbrey equation . Taking into account the diameter of the electrode (d = 10 mm) and the other technical parameters , this equation can be written as: Δm = 1.03 Δf. According to this a layer mass increase can be estimated. Since measurements have been performed in solution, these values are estimated due to unknown amount of bound water.
All electrochemical measurements were carried out in a 1 mL cell using an Ag/AgCl/1 M KCl reference (Microelectrodes, Inc., Bedford, USA) and Pt-wire counter electrode. The working electrodes were modified gold wires (diameter 0.5 mm) obtained from Goodfellow (Bad Nauheim, Germany) which are modified according to the procedures described above. Cyclic voltammetric experiments were carried out with CH Instruments CHI 660D device (Austin Texas, USA). Scan rates were varied between 0.01 and 50 V/s, but a scan rate of 100 mV/s was normally used. The potential range has been chosen between -0.3 and +0.3 V vs. Ag/AgCl. Data analysis has been performed using CHI 660D (Austin Texas, USA) software.
List of abbreviations
- APTES :
(4-aminopropyl triethoxy silane)
- CV :
- cyt c :
- D effective :
(effective diffusion coefficient)
- DET :
(direct electron transfer)
- DLS :
(dynamic light scattering)
- DNA :
- FT-IR :
(Fourier transformed infrared spectroscopy)
- IET :
(interprotein electron transfer)
- k ex :
(self-exchange rate constant)
- KPP :
(potassium phosphate buffer)
- MU :
- k s :
- MUA :
- PASA :
(polyaniline sulfonic acid)
- pH :
(p otentia h ydrogenii)
- PI :
- QCM :
(quarz crystal microbalance)
- SAM :
- SiNPs :
- TEM :
(transmission electron microscopy)
- TEOS :
This work has been financially supported by the BMBF (PNT51513). The authors also want to thank A. Kapp for his assistance in the laboratory.
- Iler RK: The Colloid Chemistry of Silica and Silicates. Cornell University Press, Ithaca, New York, 1955.Google Scholar
- Xia Y, Gates B, Yin Y; Lu Y: Monodispersed colloidal spheres - old materials with new applications. Adv Mater. 2000, 12: 693-713.View ArticleGoogle Scholar
- Stöber W, Fink A, Bohn E: Controlled growth of monodisperse silica spheres in the micron size range. J Colloid Interface Sci. 1968, 26: 62-69.View ArticleGoogle Scholar
- Bogush GH, Tracy MA, Zukoski IV CF: Preparation of monodisperse silica particles - Control of size and mass fraction. J Non-Cryst Solids. 1988, 104: 95-106.View ArticleGoogle Scholar
- Bogush GH, Zukoski IV CF: Studies of the kinetics of the precipitation of uniform silica particles through the hydrolysis and condensation of silicon alkoxides. J Colloid Interface Sci. 1991, 1-18. 142
- He P, Hu N, Rusling JF: Driving Forces for Layer-by-Layer Self-Assembly of Films of SiO2 Nanoparticles and Heme Proteins. Langmuir. 2004, 20 (3): 722-729.View ArticleGoogle Scholar
- Hilliard LR, Zhao X, Tan W: Immobilization of Oligonucleotides onto Silica Nanoparticles for DNA Hybridization Studies. Analytica Chimica Acta. 2002, 470: 51-56.View ArticleGoogle Scholar
- Sun Y, Yan F, Yang W, Zhao S, Yang W, Sun C: Effect of Silica Nanoparticles with Different Sizes on the Catalytic Activity of Glucose Oxidase. Anal Bioanal Chem. 2007, 387: 1565-1572.View ArticleGoogle Scholar
- Nam JM, Thaxton CS, Mirkin CA: Nanoparticle-based bio-bar codes for the ultrasensitive detection of proteins. Science. 2003, 301: 1884-1886.View ArticleGoogle Scholar
- Brigger I, Dubernet C, Couveur P: Nanoparticles in Cancer Diagnosis and Therapie. Adv Drug Delivery Rev. 2002, 54: 631-651.View ArticleGoogle Scholar
- Webster TJ, Schadler LS, Siegel RW, Bizios R: Mechanisms of enhanced osteoblast adhesion on nanophase alumina involve vibronectin. Tissue Eng. 2001, 7: 291-301.View ArticleGoogle Scholar
- Qhobosheane M, Santra S, Zhang P, Tan W: Biochemically Functionalized Silica Nanoparticles. The Analyst. 2001, 126: 1274-1278.View ArticleGoogle Scholar
- Tan W, Wang K, He X, Zhao XJ, Drake T, Wang L, Bagwe RP: Bionanotechnology based on silica nanoparticles. Med Res Rev. 2004, 24: 621-638.View ArticleGoogle Scholar
- Lian W, Litherland SA, Badrane H, Tan W, Wu D, Baker HV, Gulig PA, Lim DV, Jin S: Ultrasensitive detection of biomolecules with fluorescent dye-doped nanoparticles. Anal Biochem. 2004, 334: 135-144.View ArticleGoogle Scholar
- Yao G, Wang L, Wu Y, Smith J, Xu J, Zhao W, Lee E, Tan W: FloDots - luminescent nanoparticles. Anal Bioanal Chem. 2006, 385: 518-524.View ArticleGoogle Scholar
- Hsiao JK, Tsai CP, Chung TH, Hung Y, Yao M, Liu HM, Mou CY, Yang CS, Chen YC, Huang DM: Mesoporous silica nanoparticles as a delivery system of gadolinium for effective human stem cell tracking. Small. 2008, 4: 1445-1452.View ArticleGoogle Scholar
- Grant SA, Weilbaecher C, Lichlyter D: Development of a protease biosensor utilizing silica nanobeads. Sens Actuators B. 2007, B121: 482-489.View ArticleGoogle Scholar
- Feng J, Li Y, Yang M: Conjugated polymer-grafted silica nanoparticles for the sensitive detection of TNT. Sens Actuators B. 2010, 145: 438-443.View ArticleGoogle Scholar
- Dronov R, Kurth DG, Möhwald H, Scheller FW, Lisdat F: A self-assembled cytochrome c/xanthine oxidase multilayer arrangement on gold. Electrochim Acta. 2007, 53: 1107-1113.View ArticleGoogle Scholar
- Grabar KC, Allison KJ, Baker BE, Bright RM, Brown KR, Freeman RG, Fox AP, Keating CD, Musick MD, Natan MJ: Two dimensional arrays of colloidal Au particles - A flexible approach to macroscopic metal surfaces. Langmuir. 1996, 12: 2353-2361.View ArticleGoogle Scholar
- Riklin A, Willner I: Glucose and Acetylcholine Sensing Multilayer Enzyme Electrodes of Controlled Enzyme Layer Thickness. Anal Chem. 1995, 67 (22): 4118-4126.View ArticleGoogle Scholar
- Qi YM, Honma I, Ichihara M, Zhou H: Layer-by-Layer Fabrication and Characterization of Gold-Nanoparticle/Myoglobin Nanocomposite Films. Adv Funct Mater. 2006, 16: 377-386.View ArticleGoogle Scholar
- Spricigo R, Dronov R, Rajagolan KV, Lisdat F, Leimkühler S, Scheller FW, Wollenberger U: Electrocatalytical functional multilayer assembly of sulfite oxidase and cytochrome c. Soft Matter. 2008, 4: 972-978.View ArticleGoogle Scholar
- Lisdat F, Dronov R, Möhwald H, Scheller FW, Kurth D: Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains. Chem Commun. 2009: 277-283.
- He P, Hu N: Electrocatalytic properties of heme proteins in layer-by-layer films assembled with SiO2 nanoparticles. Elelctroanalysis. 2003, 16: 1122-1131.View ArticleGoogle Scholar
- Katz E, Willner I: Integrated nanoparticle-biomolecule hybrid systems: Synthesis, properties and applications. Angew Chem Int Ed. 2004, 43: 6042-6108.View ArticleGoogle Scholar
- Phadtare S, Vinod VP, Muhopadhyay K, Kumar A, Rao M, Shaudhari RV, Sastry M: Immobilization and biocatalytic activity of fungal protease on gold nanoparticle loaded zeolite microspheres. Biotechnol Bioeng. 2004, 85: 629-637.View ArticleGoogle Scholar
- Satishkumar R, Vertegel A: Charge-Directed Targeting of Antimicrobial Protein-nanoparticle Conjugates. Biotechnol Bioeng. 2008, 100: 403-412.View ArticleGoogle Scholar
- Zhao JG, Henkens RW, Al E: Direct-electron transfer at horseradish peroxidase-colloidal gold modified electrodes. J Electroanal Chem. 1992, 327: 109-119.View ArticleGoogle Scholar
- Beissenhirtz MK, Scheller FW, Lisdat F: A Superoxide Sensor Based on a Multilayer Cytochrome c electrode. Anal Chem. 2004, 76 (16): 4665-4671.View ArticleGoogle Scholar
- Beissenhirtz MK, Scheller FW, Stocklein WFM, Kurth DG, Mohwald H, Lisdat F: Electroactive Cytochrome c Multilayers within a Polyelectrolyte Assembly. Angew Chem Int Edit. 2004, 43: 4357-4360.View ArticleGoogle Scholar
- Dronov R, Kurth DG, Möhwald H, Scheller FW, Lisdat F: Communication in a Protein Stack: Electron Transfer between Cytochrome c and Bilirubin Oxidase within a polyelectrolyte Multilayer. Angew Chem. 2008, 120 (16): 3042-3045.View ArticleGoogle Scholar
- Dronov R, Kurth DG, Möhwald H, Spricigo R, Leimkühler S, Wollenberger U, Rajagopalan KV, Scheller FW, Lisdat F: Layer-by-Layer arrangement by Protein-Protein Interaction of Sulfite Oxidase and Cytochrome c Catalyzing the Oxidation of Sulfite. J Am Chem Soc. 2008, 130 (4): 1122-1123.View ArticleGoogle Scholar
- Yang H, Zhu Y: A High Performance Glucose Biosensor Enhanced via Nanosized SiO2. Analytica Chimica Acta. 2005, 554: 92-97.View ArticleGoogle Scholar
- Chirea M, Garcia-Morales V, Manzares JA, Pereira C, Gulaboski R, Silva F: Electrochemical characterization of polyelectrolyte/gold nanoparticle multilayers self-assembled on Gold electrodes. J Phys Chem B. 2005, 109: 21808-21817.View ArticleGoogle Scholar
- Sun Y, Yan F, Yang W, Sun C: Multilayered construction of glucose oxidase and silica nanoparticles on Au electrodes based on layer-by-layer covalent attachment. Biomaterials. 2006, 27 (21): 4042-4049.View ArticleGoogle Scholar
- Billsten P, Wahlgren M, Arnebrant T, McGuire J, Elwing H: Structural Changes of T4 Lysozyme upon Adsorption to Silica nanoparticles Measured by Circular Dichroism. J Colloid Interface Sci. 1995, 175: 77-82.View ArticleGoogle Scholar
- Larsericsdotter H, Oscarsson S, Buijs J: Thermodynamic analysis of proteins adsorbed on silica particles: Electrostatic effects. J Colloid Interface Sci. 2001, 237: 98-103.View ArticleGoogle Scholar
- Picart C, Mutterer J, Richert L, Luo Y, Prestwich GF, Schaaf P, Voegle JC, Lavalle P: Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers. PNAS. 2002, 99 (20): 12531-12535.View ArticleGoogle Scholar
- Porcel C, Lavalle P, Ball V, Decher G, Senger B, Voegle JC, Schaaf P: From exponential to linear growth in polyelectrolyte multilayers. Langmuir. 2006, 22: 4376-4383.View ArticleGoogle Scholar
- Ge B, Lisdat F: Superoxide sensor based on cytochrome c immobilized on mixed-thiol SAM with a new calibration method. Anal Chim Acta. 2002, 454: 53-64.View ArticleGoogle Scholar
- Liu P, Liu WM, Xue QJ: Self-assembly of functional silanes onto nano-sized silica. Chin J Chem Phys. 2003, 16: 481-486.Google Scholar
- Schiestel T, Brunner H, Tovar GEM: Controlled Surface Functionalization of Silica Nanospheres by Covalent Conjugation Reactions and Preparation of High Density Streptavidin Nanoparticles. Journal of Nanoscience and Nanotechnology. 2004, 4: 504-511.View ArticleGoogle Scholar
- Sauerbrey G: Zeitschrift fuer Physik. 1959, 155: 206-222.View ArticleGoogle Scholar
- Teller C, Halamek J, Zeravik J, Stocklein WFM, Scheller FW: Development of a bifunctional sensor using haptenized acetylcholinesterase and application for the detection of cocaine and organophosphates. Biosens Bioelectron. 2008, 24: 111-117.View ArticleGoogle Scholar
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