Figure 10

Immunoprecipitation of the self-assembled SAPV vectors Immunoprecipitation of the self-assembled SAPV vectors (SAPV-84, SAPV-Alb5 and unmodified SAPV). Panel A – The eluates from anti-Albumin beads (left) or from anti-BCMP84 beads (right) were assayed by PCR and equal amounts of the resultant products were run on 2% agarose gel containing ethidium bromide. The gel indicates that anti-Albumin beads retain exclusively SAPV-Alb5 construct, whilst anti-BCMP84 beads precipitate SAPV-84 construct. The unmodified "empty" SAPV vector was retained by neither anti-Albumin nor anti-BCMP84 beads. Panel B – Immunoprecipitation of the self-assembled SAPV vectors (SAPV-84, SAPV-Alb5 and unmodified SAPV), data pooled from 3 experiments. The eluates from anti-Albumin beads (top panel) or from anti-BCMP84 beads (bottom panel) were assayed by PCR and quantified by agarose gel electrophoresis. Band intensities were determined using GeneSnap and fluorescent imager from SynGene (Cambridge, UK). All values shown are normalised to the strongest DNA sample in each case (marked by asterisks). Error bars represent standard deviation (n = 3). Largest eluted amounts of DNA (and therefore the corresponding SAPVs) were: SAPV-Alb5 (eluted from anti-Albumin beads) and SAPV-84 (eluted from anti-BCMP84 beads). The results confirm that anti-Albumin beads precipitate SAPV-Alb5 self-assembled construct, whilst anti-BCMP84 beads retain SAPV-84 construct.