Figure 4

Detection of the tagged SAPV on Western blots. SAPV protein vector was tagged with AFP sequence. In vitro T&T reactions were run either at different temperatures (left panel) or different amounts of DNA was added to the reactions (right panel). Detection of the tagged SAPV was done using anti-GFP Rabbit polyclonal antibody (from AbCam). The right most lane (right panel) represents T&T reaction containing 2 ug of unpurified PCR products. Second lane from the right – 2 ug of DNA was ethanol-precipitated prior to T&T, following lanes – 3 ug, 6 ug, 12 ug, 18 ug and 30 ug DNA, all were ethanol-precipitated prior to T&T.