Figure 4

The single cell analysis. Time dependent mRNA expression of single living cells was examined (a and b). The ordinate represents a common logarithm of initial mRNA copies. The nd on the ordinate indicates that the product was not detected with quantitative PCR. In this case, the PCR product was not detected with ethidium bromide-stained 1% agarose gel followed by nested PCR, either. The histogram shows the number of β-actin mRNAs detected with single cell assay (c). Quantitative PCR was performed with standard curves using DNA fragments from rat β-actin.