Figure 2

A. Agarose gel electrophoresis of the soluble fractions of lysates of E. coli cells producing the wild-type (WT) and each of the mutant coat proteins (lanes 1–6). Since the particles contain host cell-derived RNA, they can be stained with ethidium bromide and visualized under UV illumination. Cellular nucleic acids are also visible as a faster-running smear. Lane 1 – wild-type, lane 2 – G13C, lane 3 – G14C; lane 4 – T15C, lane 5 – G113C, lane 6 – D114C. B. SDS gel electrophoresis of protein extracted from the same cells. Here are shown the contents of both the soluble (s) and pellet (p) fractions of crude cell lysates. Samples are labeled as in A, except for the addition of lane 0, which is a control that produces no coat protein.