Figure 4
From: Emerging Applications of Bacterial Spores in Nanobiotechnology
Detection of presence of CotC-LTB and CotC-TTFC by immunofluorescence microscopy. Sporulation of B. subtilis strains was induced by the resuspension method, and samples were taken 6 h after the onset of sporulation and analysed by immunofluorescence microscopy as described previously [46]. Samples were labelled with mouse anti-LTB antibody followed by anti-mouse IgG-TRITC conjugate (red fluorescein, Panels A & B), or rabbit anti-TTFC antibody followed by anti-rabbit IgG-FITC conjugate (green fluorescein, Panels C & D). Panels A & C, wild type spores; Panel B, isogenic spores expressing CotC-LTB); Panel D, isogenic spores expressing CotC-TTFC.