Figure 2

Agarose gel of RT-PCR of transfected cells (lanes 1 - 4) and untransfected cells (Lanes 5 - 7). Transcripts of the SNAP-tag-DARC fusion gene were detected (lane 2). 1) no RT control with SNAP-tag-DARC primers, 3) no RT control with ATPase primers, 4) RT positive control with ATPase primers (housekeeping gene), 5) RT control with SNAP-tag-DARC primers, 6) RT positive control with ATPase primers (housekeeping gene), 7) no RT control with ATPase primers, -) PCR control without primers, +) PCR control with SNAP-tag-DARC primers and total DNA from transfected cells, M) size marker.