Figure 1

Detection of immunogenic proteins on microarrays. The cell lysates are spotted onto HaloLink™ slides (shaded area) with immobilized ligand (curved lines). The HaloTag^® (pink sector) is fused to the N-terminus of the protein of interest (purple sector) and attaches covalently to the ligand. Thus, the protein of interest is covalently attached to the surface prior to screening, whereas other proteins from the cell lysate are unbound and washed away after the coupling reaction. Subsequently, primary antibodies (blue) bind to the protein of interest. Afterwards, secondary antibodies (black) conjugated with a fluorophore (yellow) bind to the respective primary antibody. The fluorescent dye is used for detection of positive spots identifying sites of potential immunogenic proteins in the process.