Figure 4
From: Microarray-based method for screening of immunogenic proteins from bacteria
SDS-PAGE of HaloTag® fusion proteins incubated with HaloTag® Alexa Fluor 488 ligand. M refers to PageRuler Plus prestained protein ladder (Fermentas) with fluorescent bands at 70 kDa and 25 kDa, The HaloTag® standard protein (HT-SP) was added as an additional size reference at 60 kDa. The bands match the expected sizes for each fusion protein. Additionally small fragments of only HaloTag® (34 kDa) are visible which might be due to early-terminated translation.