Microarray-based method for screening of immunogenic proteins from bacteria

Table 2 Overview of genes investigated.

Gene	Primer	Sequence (5'-3')	T_m [°C]	Length [bp]	immunogenic
cjaA [EMBL: CAL35100]	cjaA for	TAAAGCGATCGCCATGAAAAAAATACTTCTAAGTGT	53	840	+
	cjaA rev	ATTAGTTTAAACAATTTTTCCACCTTCAATCA	59
hisJ [EMBL: CAL34871]	hisJ for	GGCTGCGATCGCCATGAAAAAAATATTAAGCATTGCTC	61	756	+
	hisJ rev	CAAAGTTTAAACTTCTAATTCATATTTTTTAATTAAAGTA	55
pal [EMBL: CAL34284]	pal for	ATATGCGATCGCCATGAAAAAAATTCTTTTTACTTC	55	498	+
	pal rev	GCACGTTTAAACTCTTGATAATTTAAATTCAGC	54
cfrA [EMBL: CAL34884]	cfrA for	TCAGGCGATCGCCATGAAAAAAATATGTCTATCAG	52	2091	+
	cfrA rev	GATGGTTTAAACAAAGTTACCATTGATAGAAATA	52
flaC [EMBL: CAL34857]	flaC for	TGTCGCGATCGCCATGATGATCTCTGATGCAACT	59	750	+
	flaC rev	GGGGGTTTAAACTTGTAATAAATTAGCAATTTTGCTT	59
flaA [EMBL: CAL35451]	flaA for	AACTGCGATCGCCATGGGATTTCGTATTAACACC	60	1719	+
	flaA rev	CCGTGTTTAAACCTGTAGTAATCTTAAAACATTTTG	54
peb1a [EMBL: CAL35041]	peb1 for	TAAAGCGATCGCCATGGTTTTTAGAAAATCTTTGT	56	780	+
	peb1 rev	AGGGGTTTAAACTAAACCCCATTTTTTCGCTA	61
cadF [EMBL: CAL35585]	cadF for	TAAAGCGATCGCCATGAAAAAAATATTCTTATGT	50	960	+
	cadF rev	TGTCGTTTAAACTCTTAAAATAAATTTAGCAT	49
pyrC [EMBL: CAL34413]	pyrC for	GCAGGCGATCGCCATGAAACTTAAAAATCCTTTAG	53	1008	-
	pyrC rev	ACGTGTTTAAACATGTTTTAATTGAAATTTCAAA	55
pseB [EMBL: CAL35407]	pseB for	GACCGCGATCGCCATGTTTAACAAAAAAAATATCT	52	1005	-
	pseB rev	TTCTGTTTAAACAAAACCTTCAGTATGATTGAT	55
gapA [EMBL: CAL35512]	gapA for	AACAGCGATCGCCATGGCTGTAAAAGTTGCTATAAATGGT	65	999	-
	gapA rev	CACAGTTTAAACAGCCTTATTTGCAATATATACTGCCA	65
argC [EMBL: CAL34379]	argC for	GTTCGCGATCGCCATGAAAATAAAAGTTGGGATTTTAG	60	1028	-
	argC rev	CTTGGTTTAAACCAAATTTGCAAAGAATTTAAGTC	59

The gene name, accession from EMBL, primer names and sequences, their melting points, length of gene and immunogenic characteristics of the encoded protein are given

ISSN: 1477-3155