The cytotoxicity of polycationic iron oxide nanoparticles: Common endpoint assays and alternative approaches for improved understanding of cellular response mechanism

Table 3 ROS (% of control cell) and LPO induction by MNPs in SH-SY5Y cells incubated with 25 µgmL^-¹ for 1, 4, 24 and 72 h (n = 3 ± SD).

Particle	Incubation time h	ROS Assay	LPO Assay
		% DCF fluorescence	MDA nM/mg protein (Control cells: 2.620 ± 0.225)
MNP-PEI	1	99.00 (5.568)	2.702 (0.015)*
	4	115.67 (5.033)*	2.567 (0.188)
	24	121.67 (7.371)*	2.667 (0.321)
	72	113.67 (4.509)*	2.638 (0.157)
MNP-PEI-PEG	1	97.33 (6.658)	2.282 (0.341)
	4	101.00 (5.292)	2.383 (0.018)
	24	103.33 (3.512)	2.383 (0.299)
	72	102.00 (2.000)	2.651 (0.107)

ISSN: 1477-3155