Figure 4

An anti-FLAG antibody recognized the FLAG peptide in the nanofibers. Each of the nanofiber substratum containing PEG-AuNPs, FLAG-AuNPs, or AuNPs co-labeled with PEG and FLAG was incubated with an Alexa 555-conjugated anti-FLAG antibody. Fluorescence intensities inside the dashed circles were quantified using a fluorescence microscope at ×40 resolution (a). The mean fluorescence intensity of each nanofiber substratum was calculated by measuring the fluorescence intensities of 5 different areas of each nanofiber substratum (b). The fluorescence intensities indicate the amount of the recognized FLAG peptides in the nanofibers by the fluorescence-labeled anti-FLAG antibody. Error bars: SEM, n = 5.