Figure 3

Binding of aICAM-1 L and IgG L to bEnd.5 cells with varying lipid concentration. Representative FACS spectra of NIR fluorescence of TNFα-activated bEnd.5 cells after 30 min incubation at 4 °C with (a) aICAM-1 L and (b) IgG L at different liposome concentrations. (c) Mean fluorescence intensity of non-activated and TNFα-activated bEnd.5 cells after incubation with IgG L or aICAM-1 L at liposome concentrations varying from 0.0625-2 mM lipid. * = p < 0.05 vs. IgG L, ** = p < 0.05 vs. aICAM-1 L/-TNFα, t-test. n = 3 for aICAM-1 L, n = 1 for IgG L.