Binding of aICAM-1 L to bEnd.5 in the competing presence of leukocytes. Non-activated and TNFα-activated bEnd.5 cells incubated for 2 h at 37 °C with IgG L or aICAM-1 L in the presence of 0, 2.5x105 or 1x106 leukocytes/ml medium. (a-c) Typical FACS scatter plots of liposomal NIR-fluorescence vs. GRN (green) fluorescence to distinguish TNFα-activated bEnd.5 cells from RAW cells labeled with calcein (green). (d) Endothelial NIR fluorescence intensity. * = p < 0.05 vs. all groups, ANOVA with Bonferroni correction. (e) Leukocyte NIR fluorescence intensity. * = p < 0.05 vs. IgG L, ANOVA with Bonferroni correction. n = 3, except for aICAM-1 L/-TNFα n = 1.