Binding of aICAM-1 L to bEnd.5 in the competing presence of shear stress. TNFα-activated cells incubated with L or aICAM-1 L for 2 h at 37 °C and shear stress values of 0, 0.25 or 0.5 Pa. (a) Fluorescence microscopy of rhodamine lipids in liposomes adherent to bEnd.5 cells (red). Scale bar = 100 μm. (b) Cellular rhodamine fluorescence intensity quantified with FACS. * = p < 0.05 vs. all groups, ANOVA with Bonferroni correction, ** = p < 0.05 vs. IgG L, t-test. n = 2-5. (c) ICAM-1 expression at different shear stress levels. * = p < 0.05 vs. 0 Pa, ANOVA with Bonferroni correction. n = 3.