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Table 1 Properties of L, IgG L and aICAM-1 L

From: Targeting of ICAM-1 on vascular endothelium under static and shear stress conditions using a liposomal Gd-based MRI contrast agent

  antibody coupling [%] hydrodynamic diameter [nm] r1[mM-1 s-1] r2[mM-1 s-1]
NIR-liposomes     
L 2.5 ± 2.8a,b 130 ± 6b 3.3 ± 0.2b 39 ± 1b
IgG L (1:8) c 64 ± 27d 140 ± 4 2.9 22
IgG L (1:20) 54 ± 18d 140 ± 9 2.8 23
IgG L (1:40) 89 ± 46d 132 ± 10 3.2 46
IgG L (1:80) 84 ± 21d 134 ± 5 2.7 ± 0.4 38 ± 6
aICAM-1 L (1:8) 52 ± 28d 137 ± 9 2.9 27
aICAM-1 L (1:20) 66 ± 20d 133 ± 16 3.3 30
aICAM-1 L (1:40) 88 ± 24d 156 ± 0d 3.1 89
aICAM-1 L (1:80) 83 ± 9d 141 ± 2 2.7 ± 0.4 53 ± 16
Rhodamine-liposomes     
L −0.8 ± 0.8a 133 ± 5 4.2 ± 0.8 54 ± 8
aICAM-1 L (1:80) 98 ± 4d 163 ± 2 3.3 ± 0.1 53 ± 15
  1. Liposomes contained either a NIR or a rhodamine fluorophore. n = 1-5; a baseline value indicative for the inaccuracy of the protein assay; b mean ± SEM; c (1:x) the applied molar ratio of Ab: SATA; d = p < 0.05 vs. L, ANOVA with LSD correction for NIR-liposomes and t-test for rhodamine-liposomes.