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Table 1 Properties of L, IgG L and aICAM-1 L

From: Targeting of ICAM-1 on vascular endothelium under static and shear stress conditions using a liposomal Gd-based MRI contrast agent

 

antibody coupling [%]

hydrodynamic diameter [nm]

r1[mM-1 s-1]

r2[mM-1 s-1]

NIR-liposomes

    

L

2.5 ± 2.8a,b

130 ± 6b

3.3 ± 0.2b

39 ± 1b

IgG L (1:8) c

64 ± 27d

140 ± 4

2.9

22

IgG L (1:20)

54 ± 18d

140 ± 9

2.8

23

IgG L (1:40)

89 ± 46d

132 ± 10

3.2

46

IgG L (1:80)

84 ± 21d

134 ± 5

2.7 ± 0.4

38 ± 6

aICAM-1 L (1:8)

52 ± 28d

137 ± 9

2.9

27

aICAM-1 L (1:20)

66 ± 20d

133 ± 16

3.3

30

aICAM-1 L (1:40)

88 ± 24d

156 ± 0d

3.1

89

aICAM-1 L (1:80)

83 ± 9d

141 ± 2

2.7 ± 0.4

53 ± 16

Rhodamine-liposomes

    

L

−0.8 ± 0.8a

133 ± 5

4.2 ± 0.8

54 ± 8

aICAM-1 L (1:80)

98 ± 4d

163 ± 2

3.3 ± 0.1

53 ± 15

  1. Liposomes contained either a NIR or a rhodamine fluorophore. n = 1-5; a baseline value indicative for the inaccuracy of the protein assay; b mean ± SEM; c (1:x) the applied molar ratio of Ab: SATA; d = p < 0.05 vs. L, ANOVA with LSD correction for NIR-liposomes and t-test for rhodamine-liposomes.