Performance of the ILPCR assay controls. F(+): the false-positive control, which is the non-encapsulated GRIP1 reporter; F(―): the false-negative control, which is the TMV reporter encapsulated inside liposomes where the DSPE-PEG(2000)Biotin is replaced with non-binding DSPE-mPEG(2000); DL: the detection liposomes, which contain the β2-microglobin reporter encapsulated inside liposomes containing 0.5 mol% DSPE-PEG(2000)Biotin. Magenta columns: Ct values obtained when DNase I digestion was performed after rupture of the liposomes. Gray columns: Ct values obtained when DNase I digestion, with subsequent heat-deactivation of the enzyme, was performed prior to rupture of the liposomes (normal assay conditions). Orange columns: Ct values obtained in the absence of a DNase I digestion step. Measurements were performed using a Bio-Rad model CFX96 real-time PCR system.