Performance of a multiplex liposome assay. The result of a multiplex liposome assay containing a constant concentration of false-negative control liposomes in the presence of a 10,000-fold dilution series of detection liposomes. Magenta columns: Ct values of the detection liposomes (β2microglobin). Grey columns: Ct values of the false-negative control liposomes (TMV). The amplification of the TMV reporter was independent of the concentration of the detection liposomes.