Figure 2

The CSP uptake is mediated by clathrin dependent endocytosis. A. HeLa cells were incubated with CSP in a hypertonic medium containing 0.45 M sucrose for 12 hrs and were then fixed with 4% paraformaldehyde. The cells were then stained with Hoechst and were imaged using confocal microscope. B. Quantification for percentage of CSP uptake. C. HeLa cells pretreated with or without 100 μM dynasore (an inhibitor of dynamin) for 30 mins and were then incubated with CSP for 12 hrs. D. Quantification for percentage of CSP uptake with or without dynasore treatment. E. Co localization of CSP with clathrin in HeLa cells, Panel I is the enlarged image of E, panel II-IV are independent replicates of E.