Figure 5

Logarithmically scaled fluorescent and grayscale images of typical LS174t (A) and HT29 (B) colon tumor cell lines treated with non-conjugated (1) and anti-CEA (2) and anti-TAG-72 (3) conjugated NIR fluorescent HSA nanoparticles: (4) represents untreated tumor cell lines; (C) represents typical colons of healthy mice treated with non-conjugated (1) and anti-CEA (2) and anti-TAG-72 (3) conjugated NIR fluorescent HSA nanoparticles. 44 mice (each set of experiment was done with 4 mice) were anesthetized and treated with 0.1% particle dispersion in PBS, via the anus. 20 min later the colons were extensively washed with PBS and were then allowed to recover for 4 h. The colons were then removed and treated as described in the experimental part.