Figure 2

Association of PS-containing liposomes with RAW cells. a) Gd concentration of RAW cells incubated with different liposome formulations in RPMI medium as determined with ICP-MS (n = 3/group, except for PS-6-L n = 2/group). * p <0.05 vs. no L, ANOVA with Bonferroni correction. b) Representative T₁ and T₂ maps of RAW cell pellets. c) Average R₁ and d) average R₂ of cell pellets measured at 9.4 T. * p <0.05 vs. all, ANOVA with Bonferroni correction. e) CLSM images of RAW cells. Top row: in red, the fluorescence signal of the NIR-lipids present in liposomes (laser intensity 2% of maximal intensity). Bottom row: NIR signal (in red) merged with signal of labeled macrophage CD68 (in green) and cell nuclei (in blue). Scale bar = 20 μm.