Figure 4

Divalent cation dependency of liposome association with RAW cells. Representative FACS spectra for RAW cells incubated with a) PC-L or b) PS-6-L in HBSS- without Mg²⁺ and Ca²⁺, RPMI incubation medium (0.424 mM Ca²⁺ and 0.407 mM Mg²⁺) or HBSS+ with 1.26 mM Mg²⁺ and 0.90 mM Ca²⁺. c) Average fluorescence intensities for all samples (n = 4/group). * p <0.05 vs. all, ANOVA with Bonferroni correction. d) Number-weighted DLS size distributions for PC-L and PS-6-L incubated in medium with and without Ca²⁺ and Mg²⁺ (2 h, 37°C).