Figure 5

Internalization versus binding. a) Representative FACS spectra for RAW cells incubated in HBSS+ at 4°C or 37°C with PC-L or PS-6-L, or without liposomes. b) Average fluorescence intensities for all samples (n = 3/group). * p <0.05 vs. all, ANOVA with Bonferroni correction. c) CLSM of RAW cells. In red the fluorescence of NIR-lipids present in liposomes, in green macrophage CD68 and in blue cell nuclei are shown. For the NIR signal two different laser intensities are shown (2% and 25% of maximal laser intensity). Scale bar = 20 μm.