| PLGA (mg) | PVA %(w/v) | Ethyl acetate (ml) | Aqueous phase (ml) | Excess Water (ml) | Sonication Tip* | Sonication Time (s) | Stirrer Speed (rpm) | Stirrer Time (h) |
---|---|---|---|---|---|---|---|---|---|
Primary optimized conditions | 85 | 1 | 4.25 | 15 | 40 | S2 | 60 | 2000 | 4 |
First stage – 500 mg | 500 | 1 | 5 | 20 | 50 | S2 | 120 | 2000 | 4 |
Second stage – 1 g | 1000 | 1 | 10 | 40 | 60 | S14 | 120 | 3000 | 6 |
Third stage- 2 g | 2000 | 1 | 20 | 60 | 80 | S14 | 180 | 6000 | 6 |
Fourth stage – 5 g | 5000 | 1 | 50 | 150 | 200 | S14 | 300 | 8000 | 6 |