Table 2 Calculated numbers of PS NPs in stocks versus automatic count obtained from 3D confocal microscopy
NP Denomination | SPT [a] calculated size [nm] | Estimated NP number [NP/mL] | Experimental NP count [NP/mL] | SPT Fluorescence Intensity [A.U.] |
|---|---|---|---|---|
20 nm | 32 | 4.5 × 1015 | 2.2 × 1015 | 1.0 |
40 nm | 42 | 1.5 × 1015 | 5.7 × 1014 | 2.3 |
100 nm | 92 | 3.6 × 1013 | 3.7 × 1013 | 31.0 |