Figure 4

Delivery of Qdots, targeted and non-targeted to intracellular EGFR, by using liposomes-only and virus-based liposomes in medulloblastoma. Nuclei are denoted by the letter "N" and endocytic pathways are labeled by Transferrin (Tf) (green) in all confocal images. A. Double labeling of medulloblastoma (MB) cells for non-targeted Qdots (purple) delivered by liposomes-only. Scale bar equals 50 μm; B. TEM of MB cells treated with liposomes-only, which encapsulated Qdots functionalized with biotinylated antibodies for iEFGR (Qdot::iEFGR Ab complexes). Arrows point to clustered Qdots co-localized with endosomes. Scale bar equals 200 nm; C. Double labeling of MB cells for non-targeted Qdots (purple) delivered by using virus-based liposomes (VBLs.) Scale bar equals 50 μm; D. TEM of MB cells treated with VBLs that encapsulated functionalized Qdot::iEFGR Ab complexes. Arrows point to dispersed Qdots. Scale bar equals 200 nm. E. Measurement of Qdot co-localization with endosomes (black) and with cell cytosol (white) when delivered by liposomes-only or VBLs.