Figure 1

Recombinant PapMV CP proteins. (A) Amino acid sequences of the N- or C-terminus of the PapMV CP to which fusions were made. The sequence in bold corresponds to the NP_147-155 epitope from Influenza virus nucleoprotein (NP). The sequences in italics represent the flanking amino acids retained to ensure efficient processing of the epitope. (B) Bacterial lysate of the culture before induction with IPTG (first lane), after induction with IPTG (second lane) and after purification with nickel beads (third lane) of PapMV NP-12 (lanes 1–3) and PapMV NP-C (lanes 4–6). (C) Transmission electron microscope images of PapMV NP-12 and NP-C nanoparticles, respectively. (D) Size of nanoparticles and discs recorded by dynamic light scattering (DLS).