Magnetic capture from blood rescues molecular motor function in diagnostic nanodevices

Table 1 Effect of antigen (Ag) on number of actin filaments, fluorescence intensity and actomyosin motility quality in final stage of the magnetic separation procedure in Figure 3

Sample	Velocity (μm s^-1)	Fraction Motile	# of filaments	Intensity (AU)
Buffer B with Ag (n = 3)	6.44 ± 0.48	0.51 ± 0.03	628 ± 38	0.89 ± 0.11
Serum (100%) with Ag (n = 3)	5.86 ± 0.44	0.38 ± 0.05	523 ± 164	0.81 ± 0.11
Serum(100%) without Ag (n = 3)	6.26 ± 0.71	0.27 ± 0.08	27 ± 11*¹	0.22 ± 0.07*²

*p < 0.05; ¹ p = 0.0256, ² p = 0.0281 (normalized). AU: normalized arbitrary units.
Sample composed of buffer B or 100% serum with (50-60 nM) or without rIgG (Ag) was added at the first stage of this procedure. Data given as mean ± standard error of the mean from three independent experiments for each condition. Intensity values are given as normalized arbitrary units since different fluorophores were used in the different experiments. Repeated measures one-way Analysis of Variance (ANOVA) was used for statistical hypothesis testing.

ISSN: 1477-3155