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Figure 6 | Journal of Nanobiotechnology

Figure 6

From: Self-assembled nanoparticles based on modified cationic dipeptides and DNA: novel systems for gene delivery

Figure 6

Gel electrophoresis of DNA-peptide Nps. (a) Gel electrophoresis showing complexation of Arg-∆Phe and Lys-∆Phe with plasmid DNA. Lane 1 contains plasmid DNA, 2 and 3 lanes are only dipeptides, lanes 4, 5 show Arg-∆Phe-DNA Nps and Lys-∆Phe-DNA Nps respectively and lane 6 contains DNA ladder. (b) Gel electrophoresis showing resistance of DNA-peptide Nps towards DNase I treatment. Lane 1 contains plasmid DNA, lanes 4, 5 show Arg-∆Phe DNA Nps and Lys-∆Phe DNA Nps respectively and lane 8 contains DNA ladder. Nanoparticle formulations with peptide/DNA ratio of 20/1 and free DNA (4 μg) were separately incubated with 5 μl of DNase I solution (10 μg/ml in DNase/Mg2+ digestion buffer, which consisted of 50 mM Tris–HCl, pH 7.6, and 10 mM MgCl2) at 37°C for 30 min, and degradation of plasmid DNA was analyzed by electrophoresis on a 0.8% agarose gel.

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