Figure 4

Acoustic response of silicone NACPs. Brightfield image (A) and corresponding fluorescence image (B) of PVMS microparticles functionalized with biotin-TFPA and subsequently labelled with streptavidin Alexa Fluor® 488. The fluorescent image was acquired during a 250 ms exposure. The scale bars represent 50 μm. (C, D) Fluorescence images show a mixture of PVMS microparticles (red, functionalized with biotin-TFPA and streptavidin Alexa Fluor® 546) and polystyrene microparticles (green, non-biotinylated, Spherotech, 10–13 μm diameter) within a channel of an acoustofluidic device with (C) and without (D) activation of the PZT. Mixture contained a 1:7 ratio of polystyrene:PVMS microparticles. Images acquired in the absence of flow. Dashed lines are included to demarcate the channel boundaries.