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Figure 5 | Journal of Nanobiotechnology

Figure 5

From: Elastomeric microparticles for acoustic mediated bioseparations

Figure 5

Using NACPs to transport PACPs to the pressure antinode. Fluorescence images demonstrate the ability to use NACPs to transport PACPs to the pressure antinode within an acoustofluidic device. (A) As a negative control, PDMS microparticles (non-biotinylated, encapsulated with Nile Red fluorophore) were mixed with streptavidin polystyrene microparticles (green, 6 μm diameter). The lack of binding between the non-biotinylated PDMS and streptavidin polystyrene particles results in their transport to the antinode and node, respectively. (B) The high affinity between PDMS microparticles (biotinylated, encapsulated with rhodamine B fluorophore) and streptavidin polystyrene microparticles (green, 6 μm diameter) generate particle complexes that transport collectively to the pressure antinode within an ultrasound standing wave. Images acquired in the absence of flow with a 1:10 ratio of polystyrene:PDMS. Dashed lines are included to demarcate the channel boundaries. Scale bars represent 200 μm.

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