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Figure 9 | Journal of Nanobiotechnology

Figure 9

From: Investigating bioconjugation by atomic force microscopy

Figure 9

Dip pen lithography (DPN) and single molecule cut-and-paste technology by AFM. (A) Schematic representation of DPN. Reprinted with permission from [107], 1999 AAAS. The water meniscus between the AFM tip and the surface serves to transport molecules from the tip to the surface via capillary forces. (B) Schematic of the assembly of functional binding sites assembled by the single molecule cut-and-paste approach from individual α- and β-chains of a split malachite green (MG) aptamer. A complete MG binding site was formed by the β-strand transported from the storage site by the AFM cantilever tip and the α-chain at the construction site. For the β-strand transport, the AFM tip was conjugated with handle oligos, which were compatible to the handle sequence at the end of the β-chains. (C) Fluorescence microgaph of the final, single molecule cut-and-paste assembled structure, containing more than 500 aptamers. The insert shows the structural formula of the attached MG fluorophore. (B) and (C) are reproduced with permission from [113], 2012 American Chemical Society.

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