Figure 2
From: Metastatic prostate cancer cell-specific phage-like particles as a targeted gene-delivery system
Immunofluorescence microscopic demonstration of phage clone EPTHSWAT selectivity towards PC-3M cells in a co-culture of PC-3M, RWPE-1 and HT-20 cells. White arrows point PC-3M (prostate cancer) cells whereas red arrows point to HT-29 (colon cancer) cells. Phage EPTHSWAT intra-cytoplasmic and nuclear localization at 1 h post-incubation with the co-culture of PC-3M and HT-20 cells. Phage particles stained with goat anti-rabbit Alexa Fluor 488 were revealed as abundant green spots in PC-3M cells in comparison with the very scanty phage particles in HT-29 cells. The control uninfected co-culture showed no green spots.