Figure 3

Effect of nanotopography on FAK expression and actin cytoskeleton rearrangement. (A) FAK expression was evaluated by Western blot using anti-FAK antibodies in PC12 cells grown on PLL-coated glass (G-PLL), flat Titania (flat TiO₂) and nanostructured Titania (20 nm rms ns-TiO₂) in the presence and in the absence of 50 ng/mL NGF. The results are means of 2 different experiments. (B): Stress fiber organization after 48 h of culture in low serum media on different substrates and conditions: G-PLL (A), G-PLL + NGF (B), flat TiO₂ (C) and 20 nm rms ns-TiO₂ (D). Actin (red) and nucleus (blue) fluorescent staining.