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Figure 4 | Journal of Nanobiotechnology

Figure 4

From: Nitric oxide synthase mediates PC12 differentiation induced by the surface topography of nanostructured TiO2

Figure 4

Topography of TiO 2 promotes the expression of nitric oxide synthase (NOS) and cytoskeletal proteins nitration. NOS (A) and iNOS (B) expression were evaluated by Western blot analysis using anti-NOS and anti-iNOS antibodies in PC12 cells grown on PLL-coated glass (G-PLL), flat Titania (flat TiO2) and nanostructured Titania of different roughness (20 nm and 29 nm rms) in the presence and in the absence of 50 ng/mL NGF. (C) Western blot analysis using anti-nitroTyr antibodies allows to detect nitrated proteins in the Triton insoluble fraction of PC12 cells grown on PLL-coated glass (G-PLL), flat Titania (flat TiO2) and nanostructured Titania of different roughness (20 nm and 29 nm rms) in the presence and in the absence of 50 ng/mL NGF. Equal amounts of lysates (70 μg) were loaded on SDS-PAGE and probed with antibodies as indicated in the Methods section. The results are means of 3 different experiments.

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