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Figure 5 | Journal of Nanobiotechnology

Figure 5

From: Nitric oxide synthase mediates PC12 differentiation induced by the surface topography of nanostructured TiO2

Figure 5

Nitration of tubulin and actin. The expression of tubulin (A) and actin (B) were evaluated using the corresponding specific antibodies in the Triton insoluble fraction of PC12 cells grown on PLL-coated glass (G-PLL), flat Titania (flat TiO2) and nanostructured Titania of different roughness (20 nm and 29 nm rms) in the presence and in the absence of 50 ng/mL NGF. Tyr nitration of the same proteins was checked following stripping of the membrane and reprobing with anti-nitroTyr antibodies as described in the Methods section. The results are means of 2 different experiments.

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