Figure 7
Ns-TiO 2 surface nanotopography increases neurite length in human neuroblastoma cell line and triggers protein nitration. (A) bright field and SNAP-25 immunostaining of SY5Y cells plated on glass, TiO2-flat and nanostructured Titania (TiO2 20Â nm rms and TiO2 29Â nm rms); (B) bright field and SNAP-25 immunostaining of SY5Y cells plated on ns-TiO2. Insets show details of neurites. (C) neurite length distribution of cells grown on glass (G-PLL), flat TiO2 or on ns-TiO2. Kolmogorov-Smirnov: G-PLL vs. TiO2 20Â nm rms (p < 0.001), glass vs. TiO2 29Â nm rms (p < 0.001), TiO2 20Â nm rms vs. TiO2 29Â nm rms not significantly different, G-PLL vs. flat TiO2 not significantly different. (D) Histogram shows Western blot analysis using anti-nitroTyr antibodies. The results are means of 2 different experiments.