Figure 8

Involvement of ERK signaling cascade in nanostructured-induced neuritogenesis. The expression and the phosphorylation of ERK were evaluated by Western blot analysis using anti-ERK and anti-p-ERK antibodies, on PC12 cells grown on PLL-coated glass (G-PLL), flat Titania (flat TiO₂) and nanostructured Titania (20 nm rms TiO₂) in the presence and in the absence of 50 ng/mL NGF. Equal amounts of lysates (70 μg) were loaded on SDS-PAGE and probed with antibodies as indicated in the Methods section. The results are means of 3 different experiments.