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Table 1 List of nitrated cytoskeletal-related proteins in PC12 cells grown on the TiO2 surface (20 nm rms)

From: Nitric oxide synthase mediates PC12 differentiation induced by the surface topography of nanostructured TiO2

Accession

Coverage

MW

pI

Score

Description

 

%

    

P60711

94.67

41.7

5.48

1605.53

actin, cytoplasmic 1*

D3ZRN3

90.43

41.9

5.49

502.34

beta-actin-like protein 2

Q4V884

81.61

71.3

5.71

36.02

CDC16 cell division homolog

Q0V8T4

53.33

145.6

6.24

26.26

contactin-associated protein like 5-3

Q63164

75.47

514.7

5.90

265.33

dynein heavy chain 1, axonemal**

Q6IFW6

71.29

56.5

5.15

1004.55

keratin, type I cytoskeletal 10**

Q64715

71.12

198.4

4.84

61.81

microtubule associated protein**

P16884

83.21

115.3

5.85

25.20

neurofilament heavy polypeptide*

F1LSL8

77.51

288.6

6.02

108.33

protein Sptbn4 (spectrin beta chain, non-erythrocytic 4)**

Q9ERD7

68.00

50.4

4.93

373.64

tubulin beta-3 chain**

Q6PEC1

85.19

12.7

5.47

11.85

tubulin-specific chaperone A*

  1. *Proteins which have been experimentally detected as nitrated also in previous studies (reviewed in [61]); **proteins for which nitration was reported for different subunits/isoforms in previous studies as compared to the present investigation [61]; all the other proteins listed in the Table are identified as nitrated for the first time in this study.
  2. Coverage is used to determine the percent of the residues in each protein sequence that have been identified.
  3. Score is the sum of the scores of the individual peptides belonging to a protein when analyses are performed using the Sequest search engine as indicated in the Methods section. Searching for nitrated proteins against the rat NCBInr database (release February 15, 2012) was performed using the following parameters: carbamidomethylation of Cys as fixed modification, Met oxidation, Tyr nitration, Trp nitration and Ser/Thr/Tyr phosphorylation as variable modifications, trypsin (2 misses) as protease, False Discovery Rate for peptides 5% (against decoy), nitrated peptides identified amongst the Rank 1 peptides.