Flow cytometric DNA histograms of HepG2 cells. Effect of freecurcumin and CurcuEmulsome on cell population of HepG2 cells wasinvestigated for 6, 24 and 48 hours after exposure. Like freecurcumin, CurcuEmulsomes induce G2/M cell cycle arrest. However, theexpansion in population of cell at G2/M phase occurs after48 hours, rather than 24 hours as for free curcumin. Thisdelay is attributed to the gradual release of curcumin from the solidtripalmitin core of the nanocarrier inside the cell. Cells are labeledwith DAPI for detection with Pacific Blue.