Figure 1

Au-nanoprobe strategy for detection of MTBC members and mutations associated with RIF resistance. Illustration of the LAMP amplification using primers specific for MTBC members and schematic representation of the rpoB gene and positions of primers and probes. Au-nanoprobe detection strategy of LAMP products (MTBC probe) and rpoB S531L mutation: The black box indicates the sequence recognized by the MTBC-probe. The dashed box with corresponds to the sequence recognized by the mutation specific probes derived from the rpoB gene. The colorimetric assay consists of visual comparison of test solutions before and after salt induced Au-nanoprobe aggregation.