Table 4 Effect of microfluidization processing on GLA-AF recovery and physicochemical stability when buffered with 12.5 mM ammonium phosphate
Processing pressure (psi) | # Passes | Particle size (Z-ave, nm) | Pdl | Measured GLA concentration [100 ± 20 μg/ml target] | |||
|---|---|---|---|---|---|---|---|
DM | T = 6 mo | DM | T = 6 mo | DM | T = 6 mo | ||
20, 000 | 2 | 99 ± 3 | 108 ± 2 | 0.21 ± 0.01 | 0.32 ± 0.01 | 89 ± 1 | 96 ± 5 |
5 | 99 ± 4 | - | 0.25 ± 0.02 | - | - | - | |
10 | 96 ± 1 | 124 ± 5 | 0.26 ± 0.01 | 0.53 ± 0.02 | 113 ± 4 | 94 ± 2 | |
30, 000 | 2 | 99 ± 3 | 93 ± 1 | 0.21 ± 0.01 | 0.37 ± 0.02 | 89 ± 2 | 96 ± 5 |
5 | 67 ± 1 | - | 0.22 ± 0.01 | - | - | - | |
10 | 68 ± 1 | 84 ± 2 | 0.27 ± 0.02 | 0.37 ± 0.02 | 105 ± 1 | 88 ± 3 | |