Figure 3
A) Levels of GSH and B) MDA (lipid peroxidation) in AA AgNP treated and untreated cells. The oxidative status of A549 cells were assessed by quantifying GSH and MDA. Levels of the anti-oxidant GSH were measured by the GSH-Glo™ Glutatione assay (Promega, Madison, USA). AAAgNP caused a decrease in GSH (12 ± 0.24 μM) compared to untreated cells (15 ± 1.3 μM, 95% Cl = −1.0 to 6.2; p = 0.1184). The TBARS assay was utilized to quantify MDA levels as marker for lipid peroxidation induced by ROS. A significant elevation in MDA levels were found in cells exposed to AAAgNP (0.16 ± 0.023 μM) compared to the control (0.032 ± 0.016 μM, 95% Cl = −0.21 to −0.049; p = 0.0098).