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Table 1 Design of experiments: Plackett-Burman design of experiments (Minitab16) to identify optimal conditions for the ultrasonic processing of SWNT-GOx supra-molecular conjugates

From: Supramolecular glucose oxidase-SWNT conjugates formed by ultrasonication: effect of tube length, functionalization and processing time

Input Parameters

High (+)

Low (−)

Ultrasonication Time

60 minutes

5 minutes

Tube functionality

-OH

-COOH

SWNT length

~7.5 microns (long)

~3 microns (short)

Output Parameters

  

Enzyme activity

KM, kcat and kcat/KM

 

Enzyme stability

FAD absorption, λmax

 

Enzyme structure

Sum of α-helix and β-sheet

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