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Table 1 Design of experiments: Plackett-Burman design of experiments (Minitab16) to identify optimal conditions for the ultrasonic processing of SWNT-GOx supra-molecular conjugates

From: Supramolecular glucose oxidase-SWNT conjugates formed by ultrasonication: effect of tube length, functionalization and processing time

Input Parameters High (+) Low (−)
Ultrasonication Time 60 minutes 5 minutes
Tube functionality -OH -COOH
SWNT length ~7.5 microns (long) ~3 microns (short)
Output Parameters   
Enzyme activity KM, kcat and kcat/KM  
Enzyme stability FAD absorption, λmax  
Enzyme structure Sum of α-helix and β-sheet