|
vmax(mM/s)
|
KM(mM)
|
kcat(/s)
|
kcat/KM(/mM·s)
|
n
|
---|
Pristine GOx
|
0.00344 ± 0.0008
|
9.01 ± 4.1
|
17.1 ± 2.7
|
2.06 ± 0.68
|
1.86 ± 0.08
|
Sonicated GOx-5 min
|
0.00916 ± 0.0010
|
26.5 ± 7.8
|
48.7 ± 6.1
|
1.89 ± 0.35
|
1.37 ± 0.10
|
Sonicated GOx-60 min
|
0.00888 ± 0.0009
|
27.0 ± 1.6
|
44.8 ± 4.9
|
1.66 ± 0.082
|
1.46 ± 0.10
|
GOx-COOH-medium-5 min
|
0.00351 ± 0.0009
|
15.5 ± 1.7
|
17.7 ± 5.3
|
1.13 ± 0.22
|
1.14 ± 0.13
|
GOx-COOH-medium-60 min
|
0.00190 ± 0.0008
|
6.82 ± 1.9
|
8.86 ± 4.8
|
1.25 ± 0.40
|
1.82 ± 0.17
|
GOx-OH-medium-60 min
|
0.00115 ± 0.0008
|
3.11 ± 1.8
|
5.97 ± 4.8
|
1.74 ± 0.62
|
1.57 ± 0.05
|
GOx-OH-Long-60 min
|
0.00140 ± 0.0008
|
3.76 ± 2.0
|
7.01 ± 4.7
|
1.77 ± 0.34
|
2.93 ± 0.06
|
GOx-Bucky-60 min
|
0.00322 ± 0.0009
|
11.0 ± 5.7
|
16.1 ± 4.6
|
1.64 ± 0.67
|
1.40 ± 0.19
|
- (Error bars ± 95% confidence interval).