Table 2 Enzyme activity: presents and compares v max , K M , k cat , k cat /K M (a reflection of the efficiency of the enzyme) and n (the hill parameter) for pristine GOx, sonicated GOx and the various GOx-SWNT-X-L conjugates
|  | vmax(mM/s) | KM(mM) | kcat(/s) | kcat/KM(/mM·s) | n |
|---|---|---|---|---|---|
Pristine GOx | 0.00344 ± 0.0008 | 9.01 ± 4.1 | 17.1 ± 2.7 | 2.06 ± 0.68 | 1.86 ± 0.08 |
Sonicated GOx-5 min | 0.00916 ± 0.0010 | 26.5 ± 7.8 | 48.7 ± 6.1 | 1.89 ± 0.35 | 1.37 ± 0.10 |
Sonicated GOx-60 min | 0.00888 ± 0.0009 | 27.0 ± 1.6 | 44.8 ± 4.9 | 1.66 ± 0.082 | 1.46 ± 0.10 |
GOx-COOH-medium-5 min | 0.00351 ± 0.0009 | 15.5 ± 1.7 | 17.7 ± 5.3 | 1.13 ± 0.22 | 1.14 ± 0.13 |
GOx-COOH-medium-60 min | 0.00190 ± 0.0008 | 6.82 ± 1.9 | 8.86 ± 4.8 | 1.25 ± 0.40 | 1.82 ± 0.17 |
GOx-OH-medium-60 min | 0.00115 ± 0.0008 | 3.11 ± 1.8 | 5.97 ± 4.8 | 1.74 ± 0.62 | 1.57 ± 0.05 |
GOx-OH-Long-60 min | 0.00140 ± 0.0008 | 3.76 ± 2.0 | 7.01 ± 4.7 | 1.77 ± 0.34 | 2.93 ± 0.06 |
GOx-Bucky-60 min | 0.00322 ± 0.0009 | 11.0 ± 5.7 | 16.1 ± 4.6 | 1.64 ± 0.67 | 1.40 ± 0.19 |