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Table 2 Enzyme activity: presents and compares v max , K M , k cat , k cat /K M (a reflection of the efficiency of the enzyme) and n (the hill parameter) for pristine GOx, sonicated GOx and the various GOx-SWNT-X-L conjugates

From: Supramolecular glucose oxidase-SWNT conjugates formed by ultrasonication: effect of tube length, functionalization and processing time

  vmax(mM/s) KM(mM) kcat(/s) kcat/KM(/mM·s) n
Pristine GOx 0.00344 ± 0.0008 9.01 ± 4.1 17.1 ± 2.7 2.06 ± 0.68 1.86 ± 0.08
Sonicated GOx-5 min 0.00916 ± 0.0010 26.5 ± 7.8 48.7 ± 6.1 1.89 ± 0.35 1.37 ± 0.10
Sonicated GOx-60 min 0.00888 ± 0.0009 27.0 ± 1.6 44.8 ± 4.9 1.66 ± 0.082 1.46 ± 0.10
GOx-COOH-medium-5 min 0.00351 ± 0.0009 15.5 ± 1.7 17.7 ± 5.3 1.13 ± 0.22 1.14 ± 0.13
GOx-COOH-medium-60 min 0.00190 ± 0.0008 6.82 ± 1.9 8.86 ± 4.8 1.25 ± 0.40 1.82 ± 0.17
GOx-OH-medium-60 min 0.00115 ± 0.0008 3.11 ± 1.8 5.97 ± 4.8 1.74 ± 0.62 1.57 ± 0.05
GOx-OH-Long-60 min 0.00140 ± 0.0008 3.76 ± 2.0 7.01 ± 4.7 1.77 ± 0.34 2.93 ± 0.06
GOx-Bucky-60 min 0.00322 ± 0.0009 11.0 ± 5.7 16.1 ± 4.6 1.64 ± 0.67 1.40 ± 0.19
  1. (Error bars ± 95% confidence interval).
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Journal of Nanobiotechnology

ISSN: 1477-3155

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