Figure 3

Detection of m⁶A in native mRNA. (a) The section of the native mRNA containing the m⁶A modification sequence is shown in black with the potential site of methylation at position 905 shown in red and unmodified A at position 903 shown in dark yellow. The methyltransferase recognition consensus sequence is indicated in brown where R represents a purine, and H is a non-guanine base. (b) The main plot shows IPD distribution for phospholinked T incorporations across position 905 in native mRNA (red) and the in vitro transcribed control (blue), the Inset shows IPD distributions obtained at sequence position 903 in native mRNA (dark yellow) and the in vitro transcribed control (blue). (c) Pulse width (PW) distributions for phospholinked T incorporations across from the consensus site for m⁶A modification (position 905; data obtained with native mRNA and in vitro transcribed control shown in red and blue, respectively). Inset: PW distribution for phospholinked T incorporations at the unmodified adenosine control at position 903 (data obtained with native mRNA and in vitro transcribed control shown in dark yellow and blue, respectively).