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Figure 3 | Journal of Nanobiotechnology

Figure 3

From: Polyamidoamine dendrimer impairs mitochondrial oxidation in brain tissue

Figure 3

G5-NH 2 (0.1 mg/ml, 30 min) induces distinguishable mitochondrial membrane (Ψ MITO ) depolarization in astroglial and neuronal cells as monitored with the fluorescent rhodamine-123 indicator. A: Co-localization (yellow cells pointed by blue arrows) of astroglial cells stained with the astroglia-specific marker SR101 (red) with the ΨMITO depolarization indicator rhodamine-123 (green). B: Representative serial images showing ΨMITO depolarization in the circled cells in the CA3 stratum pyramidale (p) and CA3 stratum radiatum (r) areas of the rat hippocampal slice. Scale bar: 50 μm. C: Fluorescence-time plots of astroglial (Left) and neuronal (Right) cells circled in B. D: Statistical evaluation of astroglial vs. neuronal effects of G5-NH2 on ΨMITO depolarization dynamics: ΨMITO depolarization duration (Left) and percent of cells showing transient and lasting ΨMITO depolarization (Right). Asterisks represent significant differences at p < 0.05 level.

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