Figure 1From: Structure, assembly and dynamics of macromolecular complexes by single particle cryo-electron microscopyPreparation of purified molecular complexes for electron microscopy. In negative stain the specimen is adsorbed on a carbon film, embedded in a layer of heavy metal salts and dried. In frozen hydrated conditions the molecules are embedded in a thin layer of vitrified buffer suspended in a hole of the carbon film. Corresponding electron micrographs are shown (right panels). The bar represents 50 nm.Back to article page